CHIP/STUB1 Antibody

Western Blot: CHIP Antibody [NB300-982] - (0.3ug/ml) Analysis of Human Muscle lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CHIP/STUB1 Antibody Summary

• Description: Novus Biologicals Goat CHIP/STUB1 Antibody (NB300-982) is a polyclonal antibody validated for use in WB and ELISA. Anti-CHIP/STUB1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DAFISENGWVEDY corresponding to C-Terminus according to NP_005852.2.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: STUB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 35 kDa band observed in human muscle lysates (calculated MW of 34.9 kDa band according to NP_005852.2).
• Control:

  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CHIP/STUB1 Antibody

• Antigen NY-CO-7
• Carboxy terminus of Hsp70-interacting protein
• CHIP
• CHIPSTIP1 homology and U box-containing protein 1
• CLL-associated antigen KW-8
• E3 ubiquitin-protein ligase CHIP
• EC 6.3.2.-
• heat shock protein A binding protein 2 (c-terminal)
• HSPABP2
• NY-CO-7
• SDCCAG7
• serologically defined colon cancer antigen 7
• STIP1 homology and U-box containing protein 1
• STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase
• STUB1
• UBOX1

Background

STUB1 modulates the activity of several chaperone complexes, including Hsp70, Hsc70 and Hsp90. It has E3 ubiquitin protein ligase activity and targets misfolded chaperone substrates towards proteasomal degradation. STUB1 mediates transfer of non-canonical short ubiquitin chains to HSPA8 that have no effect on HSPA8 degradation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHIP/STUB1 Antibody (NB300-982)(1)

Publication using NB300-982

• Jiang J, Ballinger CA, Wu Y et al. CHIP is a U-box-dependent E3 ubiquitin ligase: identification of Hsc70 as a target for ubiquitylation. J Biol Chem 2001-11-16 [PMID: 11557750]

FAQs for CHIP/STUB1 Antibody (NB300-982). (Showing 1 - 1 of 1 FAQ).

1. In researching our products for safety information I ran across one of your products (Product number IMG3137) which I need clarification. According to our documentation, this product contains Tris-saline, 0.02% Sodium azide, 0.5% BSA and is a liquid. I am
   • With regard to product IMG-3137 (now NB300-982), the molarity of the Tris-saline solution is as follows: 20mM Tris, 150mM NaCl

Bioinformatics

• Gene Symbol: STUB1
• Entrez:
  • 10273(Human)
  • 56424(Mouse)
• Uniprot:
  • NP_005852.(Human)