Recombinant Human UbcH5a/UBE2D1 Protein, CF


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Product Details

Reactivity HuSpecies Glossary
Applications Enzyme Activity

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Recombinant Human UbcH5a/UBE2D1 Protein, CF Summary

Details of Functionality
Recombinant Human UbcH5a/UBE2D1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5a/UBE2D1 concentration of 0.1-1 μM.
E. coli-derived human UbcH5a/UBE2D1 protein
Accession #
Protein/Peptide Type
Recombinant Enzymes
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.


Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
E2-616 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human UbcH5a/UBE2D1 Protein, CF

  • E2(17)KB1
  • Stimulator of Fe transportUBC4/5 homolog
  • UBC4/5
  • UBC5A
  • UbcH5
  • UbcH5a
  • UBE2D1
  • Ubiquitin carrier protein D1
  • ubiquitin-conjugating enzyme E2 D1
  • Ubiquitin-conjugating enzyme E2(17)KB 1
  • Ubiquitin-conjugating enzyme E2-17 kDa 1
  • ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)
  • Ubiquitin-protein ligase D1


Ubiquitin-conjugating Enzyme H5a (UbcH5a), also known as Ubiquitin-conjugating Enzyme E2D 1 (UBE2D1), is a ubiquitously expressed protein that is related to Stimulator of Iron Transport (SFT) (1,2). Human UbcH5a/UBE2D1 has a predicted molecular weight of 17 kDa and shares 89% and 88% amino acid (aa) sequence identity with the related family members UbcH5b and UbcH5c, respectively (3). Human UbcH5a/UBE2D1 shares 100% aa sequence identity with the mouse and rat orthologs. UbcH5a/UBE2D1 has a conserved E2 catalytic core domain that contains an active site cysteine residue, and it interacts with a variety of HECT and RING finger Ubiquitin ligases (E3) to mediate the ubiquitination of specific target proteins (4). UbcH5a/UBE2D1 interacts with the E3, E6-AP, to conjugate Ubiquitin to the tumor suppressor, p53 (1). Additional protein targets of UbcH5a/UBE2D1 include c-Fos, RIP1, and HIF-1 (5,6). Pathologically, UbcH5a/UBE2D1 is implicated in protein degradation during cancer and immune responses (7).

  1. Scheffner, M. et al. (1994) Proc. Natl. Acad. Sci. USA 91:8797.
  2. Gehrke, S. et al. (2003) Blood 101:3288.
  3. Jensen, J. et al. (1995) J. Biol. Chem. 270:30408.
  4. Lorick, K. et al. (2005) Methods Enzymol. 398:54.
  5. Stancovski, I. et al. (1995) Mol. Cell Biol. 15:7106.
  6. Dynek, J. et al. (2010) EMBO J. 29:4128.
  7. Vanni, E. et al. (2012) J. Virol. 86:6323.

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Publications for UbcH5a/UBE2D1 (E2-616)(35)

We have publications tested in 6 confirmed species: Human, Mouse, Bacteria - E. Coli, N/A, Parasite - Plasmodium falciparum, Virus - HPV.

We have publications tested in 3 applications: Bioassay, Enzyme Assay, Ubiquitination.

Filter By Application
Enzyme Assay
All Applications
Filter By Species
Bacteria - E. Coli
Parasite - Plasmodium falciparum
Virus - HPV
All Species
Showing Publications 1 - 10 of 35. Show All 35 Publications.
Publications using E2-616 Applications Species
X Li, M Guo, L Cai, T Du, Y Liu, HF Ding, H Wang, J Zhang, X Chen, C Yan Competitive ubiquitination activates the tumor suppressor p53 Cell Death Differ., 2019;0(0):. 2019 [PMID: 31796886] (Bioassay, Human) Bioassay Human
TC Ting, M Goralski, K Klein, B Wang, J Kim, Y Xie, D Nijhawan Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15 Cell Rep, 2019;29(6):1499-1510.e6. 2019 [PMID: 31693891] (Bioassay, Human) Bioassay Human
J Sitz, SA Blanchet, SF Gameiro, E Biquand, TM Morgan, M Galloy, J Dessapt, EG Lavoie, A Blondeau, BC Smith, JS Mymryk, CA Moody, A Fradet-Tur Human papillomavirus E7 oncoprotein targets RNF168 to hijack the host DNA damage response Proc. Natl. Acad. Sci. U.S.A., 2019;116(39):19552-19562. 2019 [PMID: 31501315] (Bioassay, Virus - HPV) Bioassay Virus - HPV
MCJ Yip, AFA Keszei, Q Feng, V Chu, MJ McKenna, S Shao Mechanism for recycling tRNAs on stalled ribosomes Nat. Struct. Mol. Biol., 2019;0(0):. 2019 [PMID: 31011209] (Ubiquitination, Human) Ubiquitination Human
G Lu, J Yi, A Gubas, YT Wang, Y Wu, Y Ren, M Wu, Y Shi, C Ouyang, HWS Tan, T Wang, L Wang, ND Yang, S Deng, D Xia, RH Chen, SA Tooze, HM Shen Suppression of autophagy during mitosis via CUL4-RING ubiquitin ligases-mediated WIPI2 polyubiquitination and proteasomal degradation Autophagy, 2019;0(0):1-18. 2019 [PMID: 30898011] (Bioassay) Bioassay
OE Akande, PK Damle, M Pop, NE Sherman, BB Szomju, LV Litovchick, SR Grossman DBC1 Regulates p53 Stability via Inhibition of CBP-Dependent p53 Polyubiquitination Cell Rep, 2019;26(12):3323-3335.e4. 2019 [PMID: 30893604] (Bioassay) Bioassay
Y Dong, S Zhang, Z Wu, X Li, WL Wang, Y Zhu, S Stoilova-M, Y Lu, D Finley, Y Mao Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome Nature, 2018;0(0):. 2018 [PMID: 30479383] (Bioassay, Human) Bioassay Human
ME Matyskiela, S Couto, X Zheng, G Lu, J Hui, K Stamp, C Drew, Y Ren, M Wang, A Carpenter, CW Lee, T Clayton, W Fang, CC Lu, M Riley, P Abdubek, K Blease, J Hartke, G Kumar, R Vessey, M Rolfe, LG Hamann, PP Chamberlai SALL4 mediates teratogenicity as a thalidomide-dependent cereblon substrate Nat. Chem. Biol., 2018;0(0):. 2018 [PMID: 30190590] (Ubiquitination, Human) Ubiquitination Human
P Amin, M Florez, A Najafov, H Pan, J Geng, D Ofengeim, SA Dziedzic, H Wang, VJ Barrett, Y Ito, MJ LaVoie, J Yuan Regulation of a distinct activated RIPK1 intermediate bridging complex I and complex II in TNF?-mediated apoptosis Proc. Natl. Acad. Sci. U.S.A., 2018;115(26):E5944-E5953. 2018 [PMID: 29891719] (Bioassay) Bioassay
S Ito, A Ueno, T Ueda, H Nakagawa, H Taniguchi, N Kayukawa, A Fujihara-I, F Hongo, K Okihara, O Ukimura CNPY2 inhibits MYLIP-mediated AR protein degradation in prostate cancer cells Oncotarget, 2018;9(25):17645-17655. 2018 [PMID: 29707137] (Bioassay) Bioassay
Show All 35 Publications.

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Gene Symbol UBE2D1