Recombinant Human UbcH5a/UBE2D1 Protein, CF

Images

 
There are currently no images for UbcH5a/UBE2D1 (E2-616).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human UbcH5a/UBE2D1 Protein, CF Summary

Details of Functionality
Recombinant Human UbcH5a/UBE2D1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5a/UBE2D1 concentration of 0.1-1 μM.
Source
E. coli-derived human UbcH5a/UBE2D1 protein
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
UBE2D1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
E2-616 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human UbcH5a/UBE2D1 Protein, CF

  • E2(17)KB1
  • Stimulator of Fe transportUBC4/5 homolog
  • UBC4/5
  • UBC5A
  • UbcH5
  • UbcH5a
  • UBCH5AEC 6.3.2.19
  • UBCH5SFT
  • UBE2D1
  • Ubiquitin carrier protein D1
  • ubiquitin-conjugating enzyme E2 D1
  • Ubiquitin-conjugating enzyme E2(17)KB 1
  • Ubiquitin-conjugating enzyme E2-17 kDa 1
  • ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)
  • Ubiquitin-protein ligase D1

Background

Ubiquitin-conjugating Enzyme H5a (UbcH5a), also known as Ubiquitin-conjugating Enzyme E2D 1 (UBE2D1), is a ubiquitously expressed protein that is related to Stimulator of Iron Transport (SFT) (1,2). Human UbcH5a/UBE2D1 has a predicted molecular weight of 17 kDa and shares 89% and 88% amino acid (aa) sequence identity with the related family members UbcH5b and UbcH5c, respectively (3). Human UbcH5a/UBE2D1 shares 100% aa sequence identity with the mouse and rat orthologs. UbcH5a/UBE2D1 has a conserved E2 catalytic core domain that contains an active site cysteine residue, and it interacts with a variety of HECT and RING finger Ubiquitin ligases (E3) to mediate the ubiquitination of specific target proteins (4). UbcH5a/UBE2D1 interacts with the E3, E6-AP, to conjugate Ubiquitin to the tumor suppressor, p53 (1). Additional protein targets of UbcH5a/UBE2D1 include c-Fos, RIP1, and HIF-1 (5,6). Pathologically, UbcH5a/UBE2D1 is implicated in protein degradation during cancer and immune responses (7).

  1. Scheffner, M. et al. (1994) Proc. Natl. Acad. Sci. USA 91:8797.
  2. Gehrke, S. et al. (2003) Blood 101:3288.
  3. Jensen, J. et al. (1995) J. Biol. Chem. 270:30408.
  4. Lorick, K. et al. (2005) Methods Enzymol. 398:54.
  5. Stancovski, I. et al. (1995) Mol. Cell Biol. 15:7106.
  6. Dynek, J. et al. (2010) EMBO J. 29:4128.
  7. Vanni, E. et al. (2012) J. Virol. 86:6323.

Customers Who Viewed This Item Also Viewed...

NBP2-02042
Species: Hu
Applications: WB, Flow, ICC/IF
NBP1-89150
Species: Hu, Rt
Applications: WB, IHC, IHC-P
H00011026-M01
Species: Hu
Applications: WB, ELISA
NBP1-31302
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB600-1071
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB300-223
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Eq
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-75440
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-21037
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-31361
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-81988
Species: Hu
Applications: IHC, IHC-P
NBP1-78983
Species: Hu, Mu
Applications: WB (-), ICC/IF, IHC, IHC-P
AF9024
Species: Mu, Rt
Applications: WB, IHC
NBP2-13961
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-53246
Species: Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P

Publications for UbcH5a/UBE2D1 (E2-616)(35)

We have publications tested in 6 confirmed species: Human, Mouse, Bacteria - E. Coli, N/A, Parasite - Plasmodium falciparum, Virus - HPV.

We have publications tested in 3 applications: Bioassay, Enzyme Assay, Ubiquitination.


Filter By Application
Bioassay
(30)
Enzyme Assay
(1)
Ubiquitination
(4)
All Applications
Filter By Species
Human
(15)
Mouse
(2)
Bacteria - E. Coli
(1)
N/A
(8)
Parasite - Plasmodium falciparum
(1)
Virus - HPV
(1)
All Species
Showing Publications 1 - 10 of 35. Show All 35 Publications.
Publications using E2-616 Applications Species
X Li, M Guo, L Cai, T Du, Y Liu, HF Ding, H Wang, J Zhang, X Chen, C Yan Competitive ubiquitination activates the tumor suppressor p53 Cell Death Differ., 2019;0(0):. 2019 [PMID: 31796886] (Bioassay, Human) Bioassay Human
TC Ting, M Goralski, K Klein, B Wang, J Kim, Y Xie, D Nijhawan Aryl Sulfonamides Degrade RBM39 and RBM23 by Recruitment to CRL4-DCAF15 Cell Rep, 2019;29(6):1499-1510.e6. 2019 [PMID: 31693891] (Bioassay, Human) Bioassay Human
J Sitz, SA Blanchet, SF Gameiro, E Biquand, TM Morgan, M Galloy, J Dessapt, EG Lavoie, A Blondeau, BC Smith, JS Mymryk, CA Moody, A Fradet-Tur Human papillomavirus E7 oncoprotein targets RNF168 to hijack the host DNA damage response Proc. Natl. Acad. Sci. U.S.A., 2019;116(39):19552-19562. 2019 [PMID: 31501315] (Bioassay, Virus - HPV) Bioassay Virus - HPV
MCJ Yip, AFA Keszei, Q Feng, V Chu, MJ McKenna, S Shao Mechanism for recycling tRNAs on stalled ribosomes Nat. Struct. Mol. Biol., 2019;0(0):. 2019 [PMID: 31011209] (Ubiquitination, Human) Ubiquitination Human
G Lu, J Yi, A Gubas, YT Wang, Y Wu, Y Ren, M Wu, Y Shi, C Ouyang, HWS Tan, T Wang, L Wang, ND Yang, S Deng, D Xia, RH Chen, SA Tooze, HM Shen Suppression of autophagy during mitosis via CUL4-RING ubiquitin ligases-mediated WIPI2 polyubiquitination and proteasomal degradation Autophagy, 2019;0(0):1-18. 2019 [PMID: 30898011] (Bioassay) Bioassay
OE Akande, PK Damle, M Pop, NE Sherman, BB Szomju, LV Litovchick, SR Grossman DBC1 Regulates p53 Stability via Inhibition of CBP-Dependent p53 Polyubiquitination Cell Rep, 2019;26(12):3323-3335.e4. 2019 [PMID: 30893604] (Bioassay) Bioassay
Y Dong, S Zhang, Z Wu, X Li, WL Wang, Y Zhu, S Stoilova-M, Y Lu, D Finley, Y Mao Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome Nature, 2018;0(0):. 2018 [PMID: 30479383] (Bioassay, Human) Bioassay Human
ME Matyskiela, S Couto, X Zheng, G Lu, J Hui, K Stamp, C Drew, Y Ren, M Wang, A Carpenter, CW Lee, T Clayton, W Fang, CC Lu, M Riley, P Abdubek, K Blease, J Hartke, G Kumar, R Vessey, M Rolfe, LG Hamann, PP Chamberlai SALL4 mediates teratogenicity as a thalidomide-dependent cereblon substrate Nat. Chem. Biol., 2018;0(0):. 2018 [PMID: 30190590] (Ubiquitination, Human) Ubiquitination Human
P Amin, M Florez, A Najafov, H Pan, J Geng, D Ofengeim, SA Dziedzic, H Wang, VJ Barrett, Y Ito, MJ LaVoie, J Yuan Regulation of a distinct activated RIPK1 intermediate bridging complex I and complex II in TNF?-mediated apoptosis Proc. Natl. Acad. Sci. U.S.A., 2018;115(26):E5944-E5953. 2018 [PMID: 29891719] (Bioassay) Bioassay
S Ito, A Ueno, T Ueda, H Nakagawa, H Taniguchi, N Kayukawa, A Fujihara-I, F Hongo, K Okihara, O Ukimura CNPY2 inhibits MYLIP-mediated AR protein degradation in prostate cancer cells Oncotarget, 2018;9(25):17645-17655. 2018 [PMID: 29707137] (Bioassay) Bioassay
Show All 35 Publications.

Reviews for UbcH5a/UBE2D1 (E2-616) (0)

There are no reviews for UbcH5a/UBE2D1 (E2-616). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for UbcH5a/UBE2D1 (E2-616) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional UbcH5a/UBE2D1 Products

Bioinformatics Tool for UbcH5a/UBE2D1 (E2-616)

Discover related pathways, diseases and genes to UbcH5a/UBE2D1 (E2-616). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for UbcH5a/UBE2D1 (E2-616)

Discover more about diseases related to UbcH5a/UBE2D1 (E2-616).
 

Pathways for UbcH5a/UBE2D1 (E2-616)

View related products by pathway.

PTMs for UbcH5a/UBE2D1 (E2-616)

Learn more about PTMs related to UbcH5a/UBE2D1 (E2-616).

Blogs on UbcH5a/UBE2D1

There are no specific blogs for UbcH5a/UBE2D1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

UbcH5c/UBE2D3
E2-627-100

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human UbcH5a/UBE2D1 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol UBE2D1
Uniprot