Chimaerin 2 Overexpression Lysate (Native)

Images

 
Western Blot: Chimaerin 2 Overexpression Lysate (Adult Normal) [NBL1-09166] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Chimaerin 2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Chimaerin 2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC211342 and based on accession number NM_004067. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens chimerin (chimaerin) 2 (CHN2), transcript variant 2, mRNA.
Gene
CHN2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
53.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Chimaerin 2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Chimaerin 2 Overexpression Lysate (Native)

  • ARHGAP3beta chimerin
  • BCH
  • beta-chimaerin
  • beta-chimerin
  • chimerin (chimaerin) 2
  • MGC138360
  • Rho GTPase-activating protein 3
  • RHOGAP3
  • rho-GTPase-activating protein 3

Background

This gene is a member of the chimerin family and encodes a protein with a phorbol-ester/DAG-type zinc finger, a Rho-GAP domain and an SH2 domain. This protein has GTPase-activating protein activity that is regulated by phospholipid binding and binding of diacylglycerol (DAG) induces translocation of the protein from the cytosol to the Golgi apparatus membrane. The protein plays a role in the proliferation and migration of smooth muscle cells. Decreased expression of this gene is associated with high-grade gliomas and breast tumors, and increased expression of this gene is associated with lymphomas. Mutations in this gene have been associated with schizophrenia in men. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Transcript Variant: This variant (2) uses an alternate, upstream transcription initiation site, uses a distinct start codon and contains several additional exons, compared to variant 1. The resulting protein (isoform 2, also referred to as beta2-chimerin) has a much longer and distinct N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CHN2