CHFR Antibody

Images

 
Western Blot: CHFR Antibody [NBP1-30986] - CHFR antibody detects CHFR protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5 % SDS-PAGE, and blotted with CHFR antibody diluted by 1:500
Western Blot: CHFR Antibody [NBP1-30986] - Sample (30 ug of whole cell lysate) A: H12997 5% SDS PAGE, antibody diluted at 1:1000.
Western Blot: CHFR Antibody [NBP1-30986] - CHFR antibody detects CHFR protein by western blot analysis. Whole cell extracts (30 ug) was separated by 7.5 % SDS-PAGE, and blotted with CHFR antibody diluted by 1:500

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Xp, ZeSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CHFR Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CHFR. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Mouse (100%), Rat (100%), Bovine (100%), Zebrafish (100%), Xenopus (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHFR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Xenopus laevis (100%) and Xenopus Tropicalis (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 40% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CHFR Antibody

  • Checkpoint with forkhead and RING finger domains protein
  • checkpoint with forkhead and ring finger domains
  • E3 ubiquitin-protein ligase CHFR
  • EC 6.3.2.-
  • FLJ10796
  • FLJ33629
  • RING finger protein 196
  • RNF196RNF116

Background

A number of human cancers are sensitive to mitotic stress, implying checkpoint defects. Many proteins that contain forkhead-associated (FHA) domains are cell cycle checkpoints and CHFR is one such protein. It has a FHA and ring finger domain within its N terminus. Within its C terminus, CHFR contains a cysteine-rich region that did not display significant similarity to any protein in the GenBank database, but is highly conserved between humans and mice. It has been concluded that CHFR defines a checkpoint that delays entry into metaphase in response to mitotic stress. It has been found CpG methylation-dependent silencing of CHFR expression occurs in 45% of cancer cell lines, 40% of primary colorectal cancers, 53% of colorectal adenomas, and 30% of primary head and neck cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CHFR