CHAF1B Antibody

Samples: Whole cell lysate (1 mg forIP, 20% of IP loaded) from HeLa cells. Antibodies: Affinitypurified rabbit anti-CAF-1 p60 antibody NB100-57523 was usedfor IP at 3 ug/mg lysate. CAF-1 p60 was alsoimmunoprecipitated by rabbit anti-CAF-1 p60 antibodyNB100-57523, which recognizes a downstream epitope. Forblotting immunoprecipitated CAF-1 p60, NB100-57523 wasused at 1 ug/ml. Detection: Chemiluminescence with anexposure time of 3 seconds.

Immunohistochemistry: CHAF1B Antibody [NB100-57523] - Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-CAF-1 p60 used at a dilution of 1:200 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CHAF1B Antibody Summary

• Description: Novus Biologicals Rabbit CHAF1B Antibody (NB100-57523) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-CHAF1B Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 509 and the C-terminus (residue 559) of human chromatin assembly factor 1, subunit B (p60) using the numbering given in entry NP_005432.1 (GeneID 8208).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHAF1B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 mcg/mg
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  CHAF1B Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CHAF1B Antibody

• CAF1
• CAF-1
• CAF1ACAF-1 subunit B
• CAF1P60CAF-I 60 kDa subunit
• CAF-IP60
• chromatin assembly factor 1 subunit B
• chromatin assembly factor 1, subunit B (p60)
• Chromatin assembly factor I p60 subunit
• human chromatin assembly factor-I p60 subunit, 10
• MPHOSPH7CAF-I p60
• MPP7M-phase phosphoprotein 7

Background

Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHAF1B Antibody (NB100-57523)(3)

Publications using NB100-57523

• Azeroglu B, Khurana S, Wang SC et Al. Identification of modulators of the ALT pathway through a native FISH-based optical screen Cell Rep 2024-12-26 [PMID: 39729394]
• Lee KY, Dutta A Chk1 promotes non-homologous end joining in G1 through direct phosphorylation of ASF1A Cell reports 2021-01-26 [PMID: 33503415] (IP, Human)
• Lee K Y, Im J. S, et al. ASF1a Promotes Non-homologous End Joining Repair by Facilitating Phosphorylation of MDC1 by ATM at Double-Strand Breaks. Mol Cell 2017-10-05 [PMID: 28943310]

Bioinformatics

• Gene Symbol: CHAF1B
• Entrez:
  • 8208(Human)
• Uniprot:
  • NP_005432.1(Human)