CGRP1 Antibody (2413G) [Unconjugated]

Calcitonin was detected in immersion fixed paraffin-embedded sections of human dorsal root ganglion using Rabbit Anti-Human Calcitonin Monoclonal Antibody (Catalog # MAB83503) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Western blot shows lysates of TT human medullary thyroid cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human CGRP Monoclonal Antibody (Catalog # MAB83503) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CGRP at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western lane view shows lysates of TT human medullary thyroid cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for CGRP2 at approximately 3 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human CGRP Monoclonal Antibody (Catalog # MAB83503) . This experiment was conducted under reducing conditions and using the 2-40 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clone: 2413G
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CGRP1 Antibody (2413G) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Human CGRP synthetic peptide (Tocris Catalog # 3012)
  Accession # P06881-1
• Specificity: Detects human CGRP in direct ELISA.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CGRP1 Antibody (2413G) [Unconjugated]

• a-CGRP
• alpha-CGRP
• alpha-type CGRP
• CALCA
• calcitonin gene-related peptide I
• calcitonin/calcitonin-related polypeptide, alpha
• calcitonin; calcitonin gene-related peptide 1
• CGRP 1
• CGRP alpha
• CGRP I
• CGRP1
• CGRP-I

Background

Calcitonin gene-related peptide or CGRP is a 37-amino acid neuropeptide formed by alternative splicing of the calcitonin/CGRP gene. CGRP is a member of the calcitonin family of peptides consisting of calcitonin, amylin, adrenomedullin, adrenomedullin 2 (intermedin) and calcitonin‑receptor‑stimulating peptide. CGRP is produced in both peripheral and central neurons and induces vasodilation of a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P06881-1()