CEP250 Antibody (2739C) [Unconjugated]

Western blot shows lysates of MDA‑MB‑231 human breast cancer cells and MDA-MB-231 + Calyculin A. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human CNAP1/CEP250 Monoclonal Antibody (Catalog # MAB11339) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CNAP1/CEP250 at approximately ~250 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 2739C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CEP250 Antibody (2739C) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Phosphopeptide containing the human CEP250/CNAP1 S2421 site
  Accession # Q9BV73
• Specificity: Detects Human Phospho-CEP250/CNAP1 in direct ELISA
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CEP250 Antibody (2739C) [Unconjugated]

• 250-KD
• centrosomal Nek2-associated protein 1,250 kDa centrosomal protein
• centrosomal protein 250kDa
• centrosome associated protein
• centrosome-associated protein CEP250
• CEP2
• CEP250
• CNAP1
• C-NAP1
• MGC88542

Background

CEP250 (also known as CNAP1) is a large centrosome-associated protein that plays a crucial role in centrosome duplication and organization during cell division. Centrosomes are organelles play an essential role in cell division, cell polarity, and intracellular transport. In most animal cells, centrosomes consist of a pair of centrioles surrounded by pericentriolar material (PCM).CEP250/CNAP1 is a critical component of the PCM and is required for centrosome duplication and maturation. It forms a bridge between the centrioles and PCM and recruits other PCM components to the centrosome. Phosphorylation of CEP250/CNAP1 may regulate association/dissociation from centrosome. During M phase of mitosis, it is phosphorylated by NEK2, which may trigger the dissociation from the mitotic centrosome. This antibody detects CEP250/CNAP1 phosphorylated at Ser2421.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9BV73()