CENPK Antibody (1G2-1B10)

Images

 
Western Blot: CENPK Antibody (1G2-1B10) [H00064105-M01] - CENPK monoclonal antibody (M01), clone 1G2-1B10 Analysis of CENPK expression in HepG2.
Immunocytochemistry/ Immunofluorescence: CENPK Antibody (1G2-1B10) [H00064105-M01] - Analysis of monoclonal antibody to CENPK on HepG2 cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: CENPK Antibody (1G2-1B10) [H00064105-M01] - Detection limit for recombinant GST tagged FKSG14 is approximately 0.03ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
1G2-1B10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CENPK Antibody (1G2-1B10) Summary

Immunogen
CENPK (AAH05400.1, 1 a.a. ~ 269 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNQEDLDPDSTTDVGDVTNTEEELIRECEEMWKDMEECQNKLSLIGTETLTDSNAQLSLLIMQVKCLTAELSQWQKKTPETIPLTEDVLITLGKEEFQKLRQDLEMVLSTKESKNEKLKEDLEREQRWLDEQQQIMESLNVLHSELKNKVETFSESRIFNELKTKMLNIKEYKEKLLSTLGEFLEDHFPLPDRSVKKKKKNIQESSVNLITLHEMLEILINRLFDVPHDPYVKISDSFWPPYVELLLRNGIALRHPEDPTRIRLEAFHQ
Specificity
FKSG14 - leucine zipper protein FKSG14
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CENPK
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CENPK Antibody (1G2-1B10)

  • AF5alpha
  • CEMPK
  • CENP-KInterphase centromere complex protein 37
  • centromere protein K
  • FKSG14
  • ICEN37
  • leucine zipper protein FKSG14
  • P33
  • Protein AF-5alpha
  • SOLT
  • SoxLZ/Sox6-binding protein Solt

Background

CENPK is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CENPK Antibody (H00064105-M01) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CENPK
Entrez
Uniprot