CEBP alpha Antibody (HL1747) - Azide and BSA Free

Western Blot: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [HL1747] (NBP3-25386) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Western Blot: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C/EBP alpha antibody [HL1747] (NBP3-25386) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunocytochemistry/Immunofluorescence: CEBP alpha Antibody (HL1747) - Azide and BSA Free [NBP3-25386] - C/EBP alpha antibody [HL1747] detects C/EBP alpha protein at nucleus by immunofluorescent analysis. Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min. Green: C/EBP alpha stained by C/EBP alpha antibody [HL1747] (NBP3-25386) diluted at 1:500. Blue: Fluoroshield with DAPI .

• Reactivity: Hu, Rt, Mu, Bv, Sh
• Applications: WB, ICC/IF
• Clone: HL1747
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CEBP alpha Antibody (HL1747) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CEBP alpha Antibody (HL1747) - Azide and BSA Free (NBP3-25386) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human CEBP alpha. The exact sequence is proprietary.
• Source: HEK293
• Predicted Species: Mouse (93%), Bovine (100%), Sheep (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CEBPA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for CEBP alpha Antibody (HL1747) - Azide and BSA Free

• C/EBP alpha
• C/EBP-alpha
• CCAAT/enhancer binding protein (C/EBP), alpha
• CCAAT/enhancer-binding protein alpha
• CEBP alpha
• CEBP
• CEBPA

Background

CCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth & differentiation. The various isoforms of C/EBP (alpha, beta, gamma, delta, epsilon, zeta) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). C/EBP alpha (2) can form heterodimers with related protein C/EBP beta and gamma. C/EBP alpha directly interacts with CDK2and CDK4 and arrests cell proliferation by inhibiting these kinases (3). C/EBP alpha has been shown to bind to the promoter and modulate the expression of leptin, a protein that plays an important role in body weight homeostasis (4). C/EBP alpha also has prominent antimitotic activity and may play a role of a tumor suppressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CEBPA