CEBP alpha Antibody (15C8)

Western Blot: CEBP alpha Antibody (15C8) [NB600-1438] - Analysis of transfected 293T cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IP
• Clone: 15C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

CEBP alpha Antibody (15C8) Summary

• Background: .
• Immunogen: Synthetic peptide corresponding to residues M(1) E S A D F Y E V E P R P P(14) of mouse C/EBP alpha.
• Epitope: Amino terminal region.
• Localization: Nuclear
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CEBPA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 2 ug/ml
• Application Notes: ICC usage was reported in scientific literature (PMID: 20075868).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for CEBP alpha Antibody (15C8)

• C/EBP alpha
• C/EBP-alpha
• CCAAT/enhancer binding protein (C/EBP), alpha
• CCAAT/enhancer-binding protein alpha
• CEBP alpha
• CEBP
• CEBPA

Background

The protein encoded by this intronless gene is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. The encoded protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the encoded protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. Mutations in this gene are frequently found in acute myeloid leukemia (AML).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CEBP alpha Antibody (NB600-1438)(5)

Publications using NB600-1438

• Kang S, Lee M Beiging Modulates Inflammatory Adipogenesis in Salt-Treated and MEK6-Transfected Adipocytes Cells May 4 2021 [PMID: 34064531] (Mouse)
• Liang H, Kowalczyk P, Junco JJ et al. Differential Effects on Lung Cancer Cell Proliferation by Agonists of Glucocorticoid and PPARalpha Receptors. Mol Carcinog 2013 Apr 26 [PMID: 23625588] (WB, Human)
• Muller C, Bremer A, Schreiber S et al. Nucleolar retention of a translational C/EBPalpha isoform stimulates rDNA transcription and cell size. EMBO J. 2010 Mar. [PMID: 20075868]
• Paz-Priel I, Ghosal AK, Kowalski J et al. C/EBPalpha or C/EBPalpha oncoproteins regulate the intrinsic and extrinsic apoptotic pathways by direct interaction with NF-kappaB p50 bound to the bcl-2 and FLIP gene promoters. Leukemia. 2009 Feb. [PMID: 18987666]
• Ferrari-Amorotti, G, Keeshan, K, Zattoni, M, Guerzoni, C, Iotti, G, Cattelani, S, Donato, N J, Calabretta, B. Leukemogenesis induced by wild-type STI571-resistant BCR/ABL is potently suppressed by C/EBP{alpha}, . 108, 1353-1362. 2006 [PMID: 16670262]

Bioinformatics

• Gene Symbol: CEBPA
• Entrez:
  • 1050(Human)
  • 12606(Mouse)
  • 24252(Rat)
• Uniprot:
  • P49715(Human)