CEACAM8/CD66b Antibody (BLR111H)

Western Blot: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700] - Whole cell lysate (10 ug) from Raji, K-562, HL-60, THP-1, KG-1, MUTZ-3, SR, and Daudi cells prepared using NETN lysis buffer. Antibody: Rabbit antiCEACAM8/CD66b recombinant monoclonal antibody [BLR117H] used at 1:1000. Secondary:HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds

Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700] - Rabbit anti-CEACAM8/CD66b recombinant monoclonal antibody[BLR111H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunohistochemistry-Paraffin: CEACAM8/CD66b Antibody (BLR111H) [NBP3-14700] - Rabbit anti-CEACAM8/CD66b recombinant monoclonal antibody [BLR111H]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Immunoprecipitation: CEACAM8/CD66b Antibody [NBP3-14700] - : Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HL-60 cellsprepared using NETN lysis buffer. Antibody: Rabbit antiCEACAM8/CD66b recombinant monoclonal antibody[BLR117H] (A700-111 lot 1) used at 20 ul/mg lysate. Forblotting immunoprecipitated CEACAM8/CD66b, A700-111was used at 1:1000. Chemiluminescence with an exposuretime of 30 seconds.

• Reactivity: Hu
• Applications: WB, IHC, IP, mIF
• Clone: BLR111H
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CEACAM8/CD66b Antibody (BLR111H) Summary

• Description: Novus Biologicals Rabbit CEACAM8/CD66b Antibody (BLR111H) (NBP3-14700) is a recombinant monoclonal antibody validated for use in Multiplex Immunofluorescence, IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal
• Immunogen: residues 23-320 (ECD)
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CEACAM8
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 20ul/1mg lysate
  • Multiplex Immunofluorescence
  • Western Blot 1:1000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and
• Preservative: 0.09% Sodium Azide
• Purity: >95%

Alternate Names for CEACAM8/CD66b Antibody (BLR111H)

• Carcinoembryonic antigen CGM6
• carcinoembryonic antigen gene family member 6
• carcinoembryonic antigen-related cell adhesion molecule 8
• CD66b antigen
• CD66b
• CD67 antigen
• CD67
• CEACAM8
• CEACAM-8
• CGM6
• NCA-95
• Non-specific cross-reacting antigen NCA-95

Background

CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CEACAM8