CEACAM7 Antibody (962720) [Unconjugated]

Recombinant Human CEACAM‑7 protein was serially diluted 2-fold and captured by Mouse Anti-Human CEACAM‑7 Monoclonal Antibody (Catalog # MAB44782) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human CEACAM‑7 Monoclonal Antibody (Catalog # MAB44781) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

HEK293 human embryonic kidney cell line transfected with human CEACAM-7 and eGFP was stained with either (A) Mouse Anti-Human CEACAM‑7 Monoclonal Antibody (Catalog # MAB44782) or (B) Mouse IgG1 Isotype Control(Catalog # MAB002) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

CEACAM‑7 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Mouse Anti-Human CEACAM‑7 Monoclonal Antibody (Catalog # MAB44782) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: ELISA, Flow, IHC, CyTOF-ready
• Clone: 962720
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CEACAM7 Antibody (962720) [Unconjugated] Summary

• Immunogen: Chinese Hamster Ovary cell line, CHO-derived recombinant human CEACAM-7
  Thr36-Asn233
  Accession # Q14002
• Specificity: Detects human CEACAM-7 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CEACAM7 Antibody (962720) [Unconjugated]

• Carcinoembryonic antigen CGM 2
• Carcinoembryonic antigen CGM2
• Carcinoembryonic antigen gene family member 2
• Carcinoembryonic antigen related cell adhesion molecule 7
• CEA
• CEACAM 7
• CEACAM7
• CEACAM-7
• CGM 2
• CGM2

Background

Carcinoembryonic antigen-related cell adhesion molecule 7 (CEACAM-7), also known as CGM2, is an approximately 40 kDa GPI-anchored glycoprotein in the CEACAM family of adhesion molecules (1). Mature human CEACAM-7 consists of two Ig-like domains followed by the GPI anchor (2). Alternative splicing generates a short isoform that lacks the second Ig-like domain. CEACAM-7 is preferentially expressed on the luminal surface of epithelial cells near the mouth of colonic crypts and on pancreatic ductal epithelial cells (3, 4). It is down-regulated during colorectal adenoma progression (2-6) but can be up-regulated during the development of gastric carcinoma (7). R&D Systems in-house testing indicates that CEACAM-7 binds to CEACAM-1, consistent with the heterophilic interaction of CEACAM-1 with other CEACAM family members (1, 8, 9).

1. Tchoupa, A.K. et al. (2014) Cell Commun. Signal. 12:27.
2. Thompson, J. et al. (1994) J. Biol. Chem. 269:32924.
3. Thompson, J. et al. (1997) Cancer Res. 57:1776.
4. Scholzel, S. et al. (2000) Am. J. Pathol. 156:595.
5. Nollau, P. et al. (1997) Cancer Res. 57:2354.
6. Nollau, P. et al. (1997) Am. J. Pathol. 151:521.
7. Zhou, J. et al. (2011) World J. Surgical Oncol. 9:172.
8. Markel, G. et al. (2004) J. Immunol. 173:3732.
9. Oikawa, S. et al. (1992) Biochem. Biophys. Res. Commun. 186:881.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q14002(Human)