CEACAM5/CD66e Antibody (3G12)

Western Blot: CEACAM-5/CD66e Antibody (3G12) [NBP2-22172] - CEACAM5 Antibody (3G12) Western blot analysis using Carcino Embryonic Antigen CEA mAb against HEK293 (1) and CEA(AA: 460-600)-hIgGFc transfected HEK293 (2) cell lysate.

Immunocytochemistry/Immunofluorescence: CEACAM-5/CD66e Antibody (3G12) [NBP2-22172] - CEACAM5 Antibody (3G12) Immunofluorescence analysis of PANC-1 cells using Carcino Embryonic Antigen CEA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

ELISA: CEACAM-5/CD66e Antibody (3G12) [NBP2-22172] - CEACAM5 Antibody (3G12) Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Product Discontinued

CEACAM5/CD66e Antibody (3G12) Summary

• Immunogen: Purified recombinant fragment of human CEA expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CEACAM5
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 77 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for CEACAM5/CD66e Antibody (3G12)

• Carcinoembryonic antigen
• carcinoembryonic antigen-related cell adhesion molecule 5
• CD66e antigen
• CD66e
• CEA
• CEACAM5
• CEACAM-5
• CEACD66e
• DKFZp781M2392
• Meconium antigen 100

Background

CEACAM5 Antibody (3G12) (CEA) is synthesised during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. Antibodies to CEA are useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60 to 70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). The carcinoembryonic antigen (CEA) is a member of a large family of glycoproteins and a useful tumor marker for adenocarcinoma. Tissue specificity: Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CEACAM5