CEACAM3/CD66d Antibody [Biotin]

Human whole blood granulocytes were stained with Sheep Anti-Human CEACAM‑3/CD66d Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF4166, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

• Reactivity: Hu
• Applications: WB, Flow
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

CEACAM3/CD66d Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CEACAM‑3/CD66d isoform 1
  Lys35-Gly155
  Accession # P40198
• Specificity: Detects human CEACAM‑3/CD66d in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human (rh) CEACAM-1 is observed, 10% cross-reactivity with rhCEACAM-6 is observed, and 5% cross-reactivity with rhCEACAM-4 and rhCEACAM‑5 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CEACAM3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CEACAM3/CD66d Antibody [Biotin]

• CD66d
• CEA
• CEACAM3 carcinoembryonic antigen-related cell adhesion molecule 3
• CEACAM-3
• CGM1
• W264
• W282

Background

CEACAM-3 (carcinoembryonic antigen-related cell adhesion molecule 3; also CD66d and CGM1) is a 31‑34 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and serves as a microbial receptor for a wide range of microorganisms. Mature human CEACAM-3 is a 218 amino acid (aa) type I transmembrane glycoprotein. Its extracellular domain (aa 35‑155) shows one V-type Ig-like domain (aa 35‑142). There are two alternate splice forms. One shows a 40 aa substitution for the C-terminal 116 amino acids (aa 137‑252), while a second shows a 31 aa substitution for the C-terminal 71 amino acids. No definitive rodent CEACAM-3 has been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CEACAM3
• Uniprot:
  • P40198()