CDX2 Antibody

Product Discontinued

CDX2 Antibody Summary

• Immunogen: CDX2 antibody is made to a peptide with sequence EQLSPGGQRRNLCE corresponding to internal region according to NP_001256.1.
• Specificity: No cross-reactivity expected with CDX1 and 4
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CDX2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1 - 3 ug/mL
• Application Notes: WB: Approx. 38 kDa band observed in human kidney and liver lysates is reported in scientific literature (PMID: 12117877).
• Control:

  Human Kidney Whole Tissue Lysate (Adult Whole Normal)
  CDX2 Overexpression Lysate

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CDX2 Antibody

• caudal type homeo box transcription factor 2
• caudal type homeobox 2
• Caudal-type homeobox protein 2
• CDX2
• CDX3
• CDX-3
• CDX3caudal type homeobox transcription factor 2
• homeobox protein CDX-2

Background

CDX2 is a caudal type homeobox gene that encodes an intestine-specific transcription factor that is expressed early in intestinal development and may be involved in the regulation of proliferation and differentiation of intestinal epithelial cells. It is expressed in the nuclei of epithelial cells throughout the intestine, from duodenum to rectum. The CDX2 protein is expressed in primary and metastatic colorectal carcinomas and has also been demonstrated in the intestinal metaplasia of the stomach and intestinal-type gastric cancer, while it is not expressed in the normal gastric mucosa. Studies have shown that CDX2 is superior marker compared to CK20.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDX2 Antibody (NB100-2845)(1)

Publication using NB100-2845

• Qualtrough D, Hinoi T, Fearon E et al. Expression of CDX2 in normal and neoplastic human colon tissue and during differentiation of an in vitro model system. Gut 2002-08-01 [PMID: 12117877]

Bioinformatics

• Gene Symbol: CDX2
• Entrez:
  • 1045(Human)
  • 12591(Mouse)
  • 66019(Rat)
• Uniprot:
  • NP_001256.1(Human)