CDT1 Antibody

Western Blot: CDT1 Antibody [NB100-1098] - NB100-1098 staining of human brain lysate (RIPA buffer, 35 ug total protein per lane). Primary antibody (1 ug/ml) incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu
• Applications: WB, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

CDT1 Antibody Summary

• Immunogen: Peptide with sequence C-ARLAHQTRAEEGL corresponding to C-Terminus according to NP_112190.2.
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CDT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 60 kDa band observed in human ovary lysates (calculated MW of 60.4 kDa band according to NP_112190.2).
• Control:

  CDT1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CDT1 Antibody

• chromatin licensing and DNA replication factor 1
• DNA replication factor Cdt1
• Double parked homolog
• Double parked, Drosophila, homolog of
• DUPRIS2

Background

The CDT1 protein cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. It also binds DNA in a sequence-, strand-, and conformation-independent manner. CDT1 has been shown to interact with Geminin which inhibits both binding of the MCM complex to origins of replication and DNA-binding activity. There is some research that suggests CDT1 is a potential oncogene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDT1 Antibody (NB100-1098)(1)

Publication using NB100-1098

• Whittaker AJ, Royzman I, Orr-Weaver TL. Drosophila double parked: a conserved, essential replication protein that colocalizes with the origin recognition complex and links DNA replication with mitosis and the down-regulation of S phase transcripts. Genes Dev 2000-07-15 [PMID: 10898791]

Bioinformatics

• Gene Symbol: CDT1
• Entrez:
  • 81620(Human)
• Uniprot:
  • NP_112190.(Human)