CDT1 Antibody

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Western Blot: CDT1 Antibody [NB100-1098] - NB100-1098 staining of human brain lysate (RIPA buffer, 35 ug total protein per lane). Primary antibody (1 ug/ml) incubated for 1 hour. Detected by western blot using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CDT1 Antibody Summary

Immunogen
Peptide with sequence C-ARLAHQTRAEEGL corresponding to C-Terminus according to NP_112190.2.
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CDT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 1 - 3 ug/ml
Application Notes
WB: Approx. 60 kDa band observed in human ovary lysates (calculated MW of 60.4 kDa band according to NP_112190.2).
Control
CDT1 Overexpression Lysate
Publications
Read Publication using NB100-1098.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CDT1 Antibody

  • chromatin licensing and DNA replication factor 1
  • DNA replication factor Cdt1
  • Double parked homolog
  • Double parked, Drosophila, homolog of
  • DUPRIS2

Background

The CDT1 protein cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. It also binds DNA in a sequence-, strand-, and conformation-independent manner. CDT1 has been shown to interact with Geminin which inhibits both binding of the MCM complex to origins of replication and DNA-binding activity. There is some research that suggests CDT1 is a potential oncogene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDT1 Antibody (NB100-1098)(1)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CDT1 Antibody (NB100-1098) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional CDT1 Products

Array NB100-1098

Research Areas for CDT1 Antibody (NB100-1098)

Find related products by research area.

Blogs on CDT1

There are no specific blogs for CDT1, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

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Contact Information

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Calculators

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Bioinformatics

Gene Symbol CDT1
Entrez
Uniprot