Cdk7 Antibody [Biotin]

Western Blot: Cdk7 Antibody [Biotin] [NBP1-71799] - Whole cell lysate from HeLa (50, 15, and 5 ug). Antibody: Affinity purified, biotin-conjugated, rabbit anti-CDK7 antibody used at 0.1 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 30 seconds.

Western Blot: Cdk7 Antibody [Biotin] [NBP1-71799] - Whole cell lysate from HeLa (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 minutes.

Product Discontinued

Cdk7 Antibody [Biotin] Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 300 and the C-terminus (residue 346) of human Cyclin-Dependent Kinase 7 using the numbering given in Swiss-Prot entry P50613 (GeneID 1022).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDK7
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 0.5 - 2.5 ug/mg lysate
  • Western Blot 1:10000 - 1:25000
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 0.1 ml deionized water.

Alternate Names for Cdk7 Antibody [Biotin]

• CAK1Xenopus laevis, cdk-activating kinase)
• Cdk7
• CDK-activating kinase
• CDKN7
• Cell division protein kinase 7
• cyclin-dependent kinase 7 (homolog of Xenopus MO15 cdk-activating kinase)
• cyclin-dependent kinase 7
• EC 2.7.11
• EC 2.7.11.22
• EC 2.7.11.23
• EC 2.7.11.24
• MO15
• p39 Mo15
• serine/threonine kinase stk1,39 kDa protein kinase
• serine/threonine protein kinase 1
• serine/threonine protein kinase MO15
• STK1
• TFIIH basal transcription factor complex kinase subunit

Background

CAK (also known as CDK-activating kinase, CDK7 and cell division protein kinase 7 is a 40 kD nuclear protein that is a member of the serine/threonine family of protein kinases, CDC2/CDKX subfamily. CAK activates cyclin-associated kinases CDC2/CDK1, CDK2, CDK4, CDK6 by threonine phosphorylation and controls cell cycle progression, DNA repair, and RNA polymerase II transcription. CAK is activated by phosphorylation at Thr170 and is inactivated by phosphorylation at Ser164. CAK has been reported to interact with cyclin H and MAT1 to form the CAK complex, the CAK complex then interacts with core-TFIIH to form the TFIIH basal transcription factor. The Poly6324 antibody is useful for immunofluorescence microscopy of human CAK protein. Cyclin H, MAT1 to form CAK complex, CAK complex with core-TFIIH forms TFIIH basal transcription factor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDK7
• Entrez:
  • 1022(Human)
• Uniprot:
  • P50613(Human)