| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86666-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35288-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21225-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-30978-25ug | |||
![Western Blot: Cdk7 Antibody [NB100-581] - Detection of mouse and rat CDK7 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody NB100-581 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Cdk7-Antibody-Western-Blot-NB100-581-img0019.jpg "Western Blot: Cdk7 Antibody [NB100-581] - Detection of mouse and rat CDK7 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-CDK7 antibody NB100-581 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry: Cdk7 Antibody [NB100-581] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-fs18 CDK7 fs20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/Cdk7-Antibody-Immunohistochemistry-NB100-581-img0016.jpg)
![Western Blot: Cdk7 Antibody [NB100-581] - RIPA lysate (5 and 25 mcg/lane in A; 5 mg in B) from HeLa cells. Affinity purified rabbit anti-CDK7 antibody used at 0.1 mcg/ml for WB and at 4 mcg/5 mg lysate for IP.](http://images.novusbio.com/fullsize/Cdk7-Antibody-Western-Blot-NB100-581-img0012.jpg)
![Western Blot: Cdk7 Antibody [NB100-581] - Analysis using the Biotin conjugate of NB100-581. Detection ofHeLa (50, 15, and 5 ug). Antibody used at 1.0 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Cdk7-Antibody-Western-Blot-NB100-581-img0014.jpg)
![Western Blot: Cdk7 Antibody [NB100-581] - Analysis of Biotin conjugate of NB100-581. Whole cell lysate from HeLa (50, 15, and 5 ug). Antibody: Affinity purified, biotin-conjugated, rabbit anti-CDK7 antibody used at 0.1 ug/ml. Detection: Streptavidin-HRP and chemiluminescence with an exposure time](http://images.novusbio.com/fullsize/Cdk7-Antibody-Western-Blot-NB100-581-img0015.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Cdk7 Antibody [NB100-581] - Detection of Human CDK7 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDK7 antibody NB100-581 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Cdk7-Antibody-Western-Blot-NB100-581-img0018.jpg)
![Immunoprecipitation: Cdk7 Antibody [NB100-581] - Detection of human CDK7 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDK7 antibody NB100-581 (lot NB100-581-2) used for IP at 6 ug per reaction. CDK7 was also immunoprecipitated by a previous lot of this antibody NB100-581-1 For blotting immunoprecipitated CDK7, NB100-581 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Cdk7-Antibody-Immunoprecipitation-NB100-581-img0020.jpg)
![Western Blot: Cdk7 Antibody (BL-80-5D4) [NBP2-76409] - Detection of human CDK7 by WB of HeLa, HEK293T, Jurkat, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and HCT 116 lysate. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (NBP2-76409). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/Cdk7-Antibody-BL-80-5D4-Western-Blot-NBP2-76409-img0001.jpg "Western Blot: Cdk7 Antibody (BL-80-5D4) [NBP2-76409] - Detection of human CDK7 by WB of HeLa, HEK293T, Jurkat, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and HCT 116 lysate. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (NBP2-76409). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Immunocytochemistry: Cdk7 Antibody (BL-80-5D4) [NBP2-76409] - Detection of human CDK7 in FFPE breast carcinoma cell line MCF-7 by ICC. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (NBP2-76409). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/Cdk7-Antibody-BL-80-5D4-Immunocytochemistry-NBP2-76409-img0004.jpg)
![Immunohistochemistry: Cdk7 Antibody (BL-80-5D4) [NBP2-76409] - Detection of human CDK7 in FFPE human ovarian carcinoma by IHC. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (NBP2-76409). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/Cdk7-Antibody-BL-80-5D4-Immunohistochemistry-NBP2-76409-img0003.jpg)
![Flow Cytometry: Cdk7 Antibody (BL-80-5D4) [NBP2-76409] - Detection of human CDK7 in MOLT4 cells by flow cytometry. Antibody: Rabbit anti-CDK7 recombinant monoclonal [BL-80-3D4] NBP2-76409 (red shaded) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/Cdk7-Antibody-BL-80-5D4-Flow-Cytometry-NBP2-76409-img0005.jpg)
![Immunoprecipitation: Cdk7 Antibody (BL-80-5D4) [NBP2-76409] - Detection of human CDK7 by WB of immunoprecipitates from HeLa lysate. Antibodies: Rabbit anti-CDK7 recombinant monoclonal [BL-80-5D4] (NBP2-76409).](http://images.novusbio.com/fullsize/Cdk7-Antibody-BL-80-5D4-Immunoprecipitation-NBP2-76409-img0002.jpg)
![Western Blot: Cdk7 Antibody [NBP1-86666] - Analysis in human cell lines Caco-2 and MCF-7 using anti-CDK7 antibody. Corresponding CDK7 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.](http://images.novusbio.com/fullsize/Cdk7-Antibody-Western-Blot-NBP1-86666-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Cdk7 Antibody [NBP1-86666] - Analysis in human cell lines Caco-2 and MCF-7 using anti-CDK7 antibody. Corresponding CDK7 RNA-seq data are presented for the same cell lines. Loading control: anti-PPIB.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cdk7 Antibody [NBP1-86666] - Staining in human testis and liver tissues using NBP1-86666 antibody. Corresponding CDK7 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cdk7-Antibody-Immunohistochemistry-Paraffin-NBP1-86666-img0015.jpg)
![Immunohistochemistry-Paraffin: Cdk7 Antibody [NBP1-86666] - Staining of human cerebellum shows moderate nuclear positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Cdk7-Antibody-Immunohistochemistry-Paraffin-NBP1-86666-img0011.jpg)
![Immunohistochemistry-Paraffin: Cdk7 Antibody [NBP1-86666] - Staining of human liver shows very weak positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Cdk7-Antibody-Immunohistochemistry-Paraffin-NBP1-86666-img0012.jpg)
![Immunohistochemistry-Paraffin: Cdk7 Antibody [NBP1-86666] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Cdk7-Antibody-Immunohistochemistry-Paraffin-NBP1-86666-img0013.jpg)
![Immunohistochemistry-Paraffin: Cdk7 Antibody [NBP1-86666] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Cdk7-Antibody-Immunohistochemistry-Paraffin-NBP1-86666-img0014.jpg)
![Western Blot: Cdk7 Antibody [NBP3-35288] - Western blot analysis of various lysates using [KO Validated] Cdk7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.](http://images.novusbio.com/fullsize/nbp3-35288_rabbit-cdk7-pab-10122024120667.jpg "Western Blot: Cdk7 Antibody [NBP3-35288] - Western blot analysis of various lysates using [KO Validated] Cdk7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cdk7 Antibody [NBP3-35288] - Western Blot analysis of lysates from wild type (WT) and Cdk7 knockout (KO) 293T cells, using [KO Validated] Cdk7 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35288_rabbit-cdk7-pab-31120251744483.jpg%3Ft=1709093634)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-21225_rabbit-cdk7-pab-4520231552454.jpg)
![Western Blot: Cdk7 Antibody [NBP2-42500] - Analysis of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cdk7-Antibody-Western-Blot-NBP2-42500-img0001.jpg "Western Blot: Cdk7 Antibody [NBP2-42500] - Analysis of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![ELISA: Human Cdk7 ELISA Kit (Colorimetric) [NBP2-75178] - Samples were spiked with high concentrations of Human Cdk7 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75178_human-cdk7-elisa-kit-colorimetric-132202416232366.jpg "ELISA: Human Cdk7 ELISA Kit (Colorimetric) [NBP2-75178] - Samples were spiked with high concentrations of Human Cdk7 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Cdk7 ELISA Kit (Colorimetric) [NBP2-75178] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75178_human-cdk7-elisa-kit-colorimetric-432024916335.jpg)
![Western Blot: Cdk7 Overexpression Lysate (Adult Normal) [NBL1-09045] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cdk7.](http://images.novusbio.com/fullsize/Cdk7-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09045-img0002.jpg "Western Blot: Cdk7 Overexpression Lysate (Adult Normal) [NBL1-09045] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Cdk7.")