CDCA3 Antibody

Immunocytochemistry/Immunofluorescence: CDCA3 Antibody [NBP2-98988] - Immunofluorescence staining of CDCA3 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CDCA3 polyclonal antibody (dilution ratio: 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green)Positive staining was localized to cytoplasm.

Immunohistochemistry-Paraffin: CDCA3 Antibody [NBP2-98988] - Immunochemical staining of human CDCA3 in human colon carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: CDCA3 Antibody [NBP2-98988] - Immunochemical staining of human CDCA3 in human brain with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CDCA3 Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human CDCA3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDCA3
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 2.5 ug/mL
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for CDCA3 Antibody

• C8
• cell division cycle associated 3
• FBXO52
• Gene-rich cluster protein C8
• GRCC8cell division cycle-associated protein 3
• MGC2577
• TOME1
• TOME-1gene rich cluster, C8
• trigger of mitotic entry 1
• Trigger of mitotic entry protein 1

Background

CDCA3/TOME1 is an F-box protein and is part of the SCF complex, an ubiquitin ligase complex that promotes degradation of the mitosis-inhibitory kinase, wee1. CDCA3/TOME1 expression is cell cycle regulated by cis-promoter elements and activated at the G2/M phase. CDCA3/TOME1 levels are also regulated at the protein level via degradation by the APC (anaphase-promoting complex) during G1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDCA3