CDC5L Antibody (1D10W9)

Western Blot: CDC5L Antibody (1D10W9) [NBP3-16816] - Western blot analysis of extracts of various cell lines, using CDC5L Rabbit mAb (NBP3-16816) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry-Paraffin: CDC5L Antibody (1D10W9) [NBP3-16816] - Immunohistochemistry of paraffin-embedded human colon using CDC5L Rabbit mAb (NBP3-16816) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clone: 1D10W9
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CDC5L Antibody (1D10W9) Summary

• Description: Novus Biologicals Rabbit CDC5L Antibody (1D10W9) (NBP3-16816) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human CDC5L (Q99459). PQISDAELQEVVKVGQASEIARQTAEESGITNSASSTLLSEYNVTNNSVALRTPRTPASQDRILQEAQNLMALTNVDTPLKGGLNTPLHESDFSGVTPQRQ
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CDC5L
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for CDC5L Antibody (1D10W9)

• CDC5 (cell division cycle 5, S. pombe, homolog)-like
• CDC5 cell division cycle 5-like (S. pombe)
• CDC5
• Cdc5-like protein
• Cdc5-related protein
• CEF1
• cell division cycle 5-like protein
• dJ319D22.1 (CDC5-like protein)
• dJ319D22.1
• hCDC5
• KIAA0432
• PCDC5RPCDC5-LIKE
• Pombe cdc5-related protein

Background

CDC5L is encoded by this gene shares a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC5L