CDC42 Antibody

Images

 
Western Blot: CDC42 Antibody [NBP2-14907] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CDC42 antibody diluted at 1:500.
Immunohistochemistry-Paraffin: CDC42 Antibody [NBP2-14907] - Paraffin-embedded mouse brain. CDC42 antibody diluted at 1:500.
Western Blot: CDC42 Antibody [NBP2-14907] - Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: CDC42 Antibody [NBP2-14907] - Paraffin-embedded mouse brain. CDC42 antibody diluted at 1:500.

Product Details

Summary
Reactivity Mu, Rt, Hu, Po, Bv, Ca, Ch, Dr, Mk, Pm, Sh, Xp, ZeSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CDC42 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CDC42. The exact sequence is proprietary.
Predicted Species
Human (100%), Porcine (95%), Primate (100%), Monkey (100%), Xenopus (99%), Sheep (99%), Zebrafish (99%), Chicken (99%), Canine (100%), Bovine (100%), Drosophila (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDC42
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-14907 in the following applications:

Reactivity Notes

Rhesus Monkey (100%), Chimpanzee (100%), Xenopus laevis (99%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CDC42 Antibody

  • CDC42
  • CDC42Hs
  • cell division control protein 42 homolog
  • cell division cycle 42 (GTP binding protein, 25kDa)
  • cell division cycle 42 (GTP-binding protein, 25kD)
  • dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD))
  • dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD))
  • G25K GTP-binding protein
  • G25K
  • growth-regulating protein
  • GTP-binding protein, 25kD
  • small GTP binding protein CDC42

Background

The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDC42 Antibody (NBP2-14907) (0)

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Review for CDC42 Antibody (NBP2-14907) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: ICC.

Reviews using NBP2-14907:
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(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Wei Du
ICC Mouse 11/08/2017
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Summary

ApplicationImmunocytochemistry
Sample Testedhematopietic stem cells
SpeciesMouse
Lot39471

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Wei Du
11/08/2017
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol CDC42
Entrez
Uniprot