CDC25C Antibody

Images

 
Western Blot: CDC25C Antibody [NBP2-89147] - Analysis of Cdc25C on human skin tissue lysate using anti-Cdc25C antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: CDC25C Antibody [NBP2-89147] - Staining Cdc25C in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% ...read more
Immunohistochemistry-Paraffin: CDC25C Antibody [NBP2-89147] - Analysis of paraffin-embedded human placenta tissue using anti-Cdc25C antibody. Counter stained with hematoxylin.
Flow Cytometry: CDC25C Antibody [NBP2-89147] - Analysis of LOVO cells with Cdc25C antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor ...read more
Immunocytochemistry/ Immunofluorescence: CDC25C Antibody [NBP2-89147] - Staining Cdc25C in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% ...read more
Immunocytochemistry/ Immunofluorescence: CDC25C Antibody [NBP2-89147] - Staining Cdc25C in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% ...read more
Immunohistochemistry-Paraffin: CDC25C Antibody [NBP2-89147] - Analysis of paraffin-embedded rat brain tissue using anti-Cdc25C antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CDC25C Antibody Summary

Immunogen
Peptide
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CDC25C
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:500-1:2000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
1 x TBS (pH7.4), 0.5%BSA, 50%Glycerol.
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for CDC25C Antibody

  • CDC 25
  • CDC25
  • CDC25C
  • cell division cycle 25 homolog C (S. cerevisiae)
  • cell division cycle 25 homolog C (S. pombe)
  • cell division cycle 25C
  • Dual specificity phosphatase Cdc25C
  • EC 3.1.3.48
  • mitosis inducer CDC25
  • M-phase inducer phosphatase 3
  • phosphotyrosine phosphatase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CDC25C