CDC25C [p Ser216] Antibody

Product Discontinued

CDC25C [p Ser216] Antibody Summary

• Immunogen: Synthetic phosphopeptide derived from human Cdc25C around the phosphorylation site of Serine 216.
• Modification: p Ser216
• Marker: Mitosis Marker
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDC25C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Immunohistochemistry (IHC) analyzes of p-CDC25C (S216) pAb in paraffin-embedded human breast carcinoma tissue.
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  CDC25C Overexpression Lysate

Reactivity Notes

This antibody is reactive against Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CDC25C [p Ser216] Antibody

• CDC 25
• CDC25
• CDC25C
• cell division cycle 25 homolog C (S. cerevisiae)
• cell division cycle 25 homolog C (S. pombe)
• cell division cycle 25C
• Dual specificity phosphatase Cdc25C
• EC 3.1.3.48
• mitosis inducer CDC25
• M-phase inducer phosphatase 3
• phosphotyrosine phosphatase

Background

Cell cycle events are regulated by the sequential activation and deactivation of cyclin-dependent kinases (Cdks), including Cdk2 and Cdc2. Cdk2, in complexes with cyclin E and cyclin A, appears necessary for the onset and progression of DNA replication, while the Cdc2 kinase, in complexes with cyclin A or cyclin B, is required for the initiation of cell division. Wee 1 has been identified as a protein kinase that suppresses the entry into mitosis by mediating inhibiting tyrosine phosphorylation of Cdc2 p34. In contrast, members of the Cdc25 family of protein phosphatases function as mitotic activators by dephosphorylation of Cdc2 p34 on regulatory tyrosine and possibly threonine residues. The Cdc25 gene family consists of at least three members that share approximately 40% identity in their most conserved carboxy-terminal sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC25C
• Entrez:
  • 995(Human)