CDC25C [p Ser216] Antibody


Immunohistochemistry-Paraffin: Cdc25C Antibody [NB100-92494] - Paraffin-embedded human breast carcinoma tissue.

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CDC25C [p Ser216] Antibody Summary

Synthetic phosphopeptide derived from human Cdc25C around the phosphorylation site of Serine 216.
p Ser216
Mitosis Marker
Immunogen affinity purified
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  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Immunohistochemistry (IHC) analyzes of p-CDC25C (S216) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
CDC25C Lysate (NBL1-08996)

Reactivity Notes

This antibody is reactive against Human.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2)
0.05% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for CDC25C [p Ser216] Antibody

  • CDC 25
  • CDC25
  • CDC25C
  • cell division cycle 25 homolog C (S. cerevisiae)
  • cell division cycle 25 homolog C (S. pombe)
  • cell division cycle 25C
  • Dual specificity phosphatase Cdc25C
  • EC
  • mitosis inducer CDC25
  • M-phase inducer phosphatase 3
  • phosphotyrosine phosphatase


Cell cycle events are regulated by the sequential activation and deactivation of cyclin-dependent kinases (Cdks), including Cdk2 and Cdc2. Cdk2, in complexes with cyclin E and cyclin A, appears necessary for the onset and progression of DNA replication, while the Cdc2 kinase, in complexes with cyclin A or cyclin B, is required for the initiation of cell division. Wee 1 has been identified as a protein kinase that suppresses the entry into mitosis by mediating inhibiting tyrosine phosphorylation of Cdc2 p34. In contrast, members of the Cdc25 family of protein phosphatases function as mitotic activators by dephosphorylation of Cdc2 p34 on regulatory tyrosine and possibly threonine residues. The Cdc25 gene family consists of at least three members that share approximately 40% identity in their most conserved carboxy-terminal sequences.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol CDC25C