CDC25C Antibody (1F12)

Images

 
Western Blot: CDC25C Antibody (1F12) [NBP1-47475] - Western blot analysis using anti-CDC25C mAb against Hela(1), K562(2), PC-3(3), HEK293(4) and Raw264.7(5) cell lysate.
Immunohistochemistry-Paraffin: CDC25C Antibody (1F12) [NBP1-47475] - Immunohistochemical analysis of paraffin-embedded human Testis tissues using anti-CDC25C mouse mAb

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC-P
Clone
1F12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CDC25C Antibody (1F12) Summary

Immunogen
Purified recombinant fragment of human CDC25C expressed in E. Coli.
Marker
Mitosis Marker
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CDC25C
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Immunohistochemistry-Paraffin
Theoretical MW
58 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for CDC25C Antibody (1F12)

  • CDC 25
  • CDC25
  • CDC25C
  • cell division cycle 25 homolog C (S. cerevisiae)
  • cell division cycle 25 homolog C (S. pombe)
  • cell division cycle 25C
  • Dual specificity phosphatase Cdc25C
  • EC 3.1.3.48
  • mitosis inducer CDC25
  • M-phase inducer phosphatase 3
  • phosphotyrosine phosphatase

Background

Cdc25C is a tyrosine protein phosphatase required for progression of the cell cycle. Cdc25C activates cell cycle-specific cyclin-dependent kinases (CDKs), including cdc2, via dephosphorylation events (1,2). Cdc25C is activated via phosphorylation at Ser216 (3). In response to DNA damage and DNA replicational stress, Chk1 and Chk2 phosphorylate Cdc25C to prevent entry into mitosis (4,5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CDC25C
Entrez