CDC25A Antibody [Agarose]

Product Discontinued

CDC25A Antibody [Agarose] Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 175 and 225 of human Cell Division Cycle 25A using the numbering given in entry NP_001780.2 (GeneID 993).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDC25A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
• Application Notes: This antibody is useful for Immunoprecipitation 15 -25 mcl of gel slurry per 1 -1 mg of protein lysate or extract.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for CDC25A Antibody [Agarose]

• CDC25A
• CDC25A2
• CDC25A2-CAG isoform
• cell division cycle 25 homolog A (S. cerevisiae)
• cell division cycle 25 homolog A (S. pombe)
• cell division cycle 25A
• Dual specificity phosphatase Cdc25A
• EC 3.1.3.48
• M-phase inducer phosphatase 1

Background

Cyclin-dependent kinases are positively regulated by association with cyclins and negatively regulated by binding to inhibitory subunits. The activity of cyclin-dependent kinases is also regulated by the phosphorylation status, which is controlled by the antagonistic action of wee1 kinase and CDC25 phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, andCDC25C. These three genes function at different phases of the cell cycle. Whereas CDC25A and CDC25B are expressed throughout the cell cycle, with peak expression in G1 for CDC25A and in both G1-S-phase and G2 for CDC25B, CDC25C is predominantly expressed in G2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC25A