CDC25A Antibody (DCS-124)

Product Discontinued

CDC25A Antibody (DCS-124) Summary

• Immunogen: The immunogen for this antibody is CDC25A. The exact immunogenic sequence is proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDC25A
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000

Reactivity Notes

Human, Mouse, Rat. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 50% Glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CDC25A Antibody (DCS-124)

• CDC25A
• CDC25A2
• CDC25A2-CAG isoform
• cell division cycle 25 homolog A (S. cerevisiae)
• cell division cycle 25 homolog A (S. pombe)
• cell division cycle 25A
• Dual specificity phosphatase Cdc25A
• EC 3.1.3.48
• M-phase inducer phosphatase 1

Background

The Cdc2/cyclin B enzyme, involved in regulation of mitosis in eukaryotic cells,is subject to multiple levels of control. Among these, the regulation of the catalytic subunit by Tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B complex, while tyrosine dephosphorylation,which occurs at the onset of mitosis, directly activates the pre-MPH complex. The Cdc25 gene serves as a rate-limiting mitotic activator, apparently due to its action as the Cdc2 tyrosine phosphatase. In the absence of Cdc25,Cdc2 accumulates in a tyrosine phosphorylated state. In addition, Cdc25 proteins from a variety of species have been shown to share a low degree of sequence similarity with other tyrosine phosphatases. The Cdc25 gene family consists of at least three members that share approximately 40% identity in their most conserved carboxy-terminal sequences.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC25A