Cdc20 Antibody - BSA Free

Immunohistochemistry-Paraffin: Cdc20 Antibody [NB100-59827] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CDC20 used at a dilution of 1:500 (2ug/ml). Detection: DAB.

Immunoprecipitation: Cdc20 Antibody [NB100-59827] - Detection of human CDC20 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CDC20 antibody NB100-59827 (lot 2) used for IP at 6 ug/mg lysate. CDC20 was also immunoprecipitated by a previous lot (lot 1) of this antibody. For blotting immunoprecipitated CDC20, NB100-59827 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Po
• Applications: IHC, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Cdc20 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human cell division cycle 20 using the numbering given in entry NP_001246.2 (GeneID 991).
• Predicted Species: Porcine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDC20
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200- 1:1000
  • Immunohistochemistry-Paraffin 1:200- 1:1000
  • Immunoprecipitation 2-10 ug/mg lysate
• Application Notes: Epitope retrieval with Tris-EDTA pH9.0 is recommended.
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Cdc20 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cdc20 Antibody - BSA Free

• CDC20 cell division cycle 20 homolog (S. cerevisiae)
• CDC20 cell division cycle 20 homolog
• Cdc20
• cell division cycle 20 homolog (S. cerevisiae)
• cell division cycle protein 20 homolog
• MGC102824
• p55CDC
• p55CDCS. cerevisiae, homolog)

Background

CDC20 is a WD repeat containing cell cycle-regulator that binds to and activates the ubiquitin-ligase activity of the APC/c (anaphase-promoting complex/cyclosome). APC/c ubiquitinating activity is responsible for the degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. CDC20 has also been reported to form a complex with histone deacetylases and can function as a transcriptional repressor involved in cell cycle arrest. As a regulator of APC/c activity, CDC20 plays a key role in the spindle-assembly checkpoint.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cdc20 Antibody (NB100-59827)(2)

Publications using NB100-59827

• Matsumura F, Yamakita Y, Yamashiro S et al. Myosin phosphatase-targeting subunit 1 controls chromatid segregation. J Biol Chem 2011-03-01 [PMID: 21252232]
• Ahlskog JK, Bjork JK, Elsing AN et al. Anaphase-promoting complex/cyclosome participates in the acute response to protein-damaging stress. Mol Cell Biol 2010-12-01 [PMID: 20937767]

Bioinformatics

• Gene Symbol: CDC20
• Entrez:
  • 991(Human)
• Uniprot:
  • Q12834(Human)