Cdc14A Antibody (DCS-291)

Product Discontinued

Cdc14A Antibody (DCS-291) Summary

• Immunogen: Recombinant fragment (Human).
• Localization: Nuclear
• Specificity: Cdc14A (DCS-291)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDC14A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. Western Blot: Use at a concentration of 0.5 ug/ml. Detects a band of approximately 50 kDa. Optimal dilutions/concentrations should be determined by the end user.
• Control:

  A-431 Whole Cell Lysate

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cdc14A Antibody (DCS-291)

• CDC10 (cell division cycle 10, S. cerevisiae, homolog)
• CDC14 cell division cycle 14 homolog A (S. cerevisiae)
• CDC14 cell division cycle 14 homolog A
• cdc14
• Cdc14A1
• Cdc14A2
• dual specificity protein phosphatase CDC14A
• EC 3.1.3.16
• EC 3.1.3.48
• hCDC14

Background

Overproduction of hCdc14A leads to progressive cell death, accompanied by accumulation of pre-G1 DNA fragments and gradual elimination of cells at the G2/M transition. In 50% of the mitotic cells, multipolar mitotic spindles and misaggregated chromosomes are found. hCdc14A localizes to the centrosomes at the cell-cycle interphase stage. When the cell enters mitosis, this localization disappears suggesting that hCdc14A dissociates from the centrosomes at the G2/M transition. hCdc14A localizes to interphase centrosomes, but not to mitotic centrosomes, while hCdc14B localizes to the interphase nucleolus. Thus each isoform regulates separate cell cycle events. Cdc14A may affect cell cycle progression by its ability to dephosphorylate p53 at Ser315. This phosphatase activity is mediated by the interaction between the N-terminus of hCdc14A and the C-terminus of p53.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC14A
• Entrez:
  • 8556(Human)