CDC123 Antibody (OTI1F8)

Western Blot: CDC123 Antibody (OTI1F8) [NBP2-45600] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDC123.

Immunocytochemistry/Immunofluorescence: CDC123 Antibody (OTI1F8) [NBP2-45600] - Analysis of HeLa cells.

Immunohistochemistry: CDC123 Antibody (OTI1F8) [NBP2-45600] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Western Blot: CDC123 Antibody (OTI1F8) [NBP2-45600] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunocytochemistry/Immunofluorescence: CDC123 Antibody (OTI1F8) [NBP2-45600] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CDC123.

Immunohistochemistry: CDC123 Antibody (OTI1F8) [NBP2-45600] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: CDC123 Antibody (OTI1F8) [NBP2-45600] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Immunohistochemistry: CDC123 Antibody (OTI1F8) [NBP2-45600] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, ICC/IF, IHC, IHC-P
• Clone: OTI1F8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

CDC123 Antibody (OTI1F8) Summary

• Immunogen: Full length human recombinant protein of human CDC123(NP_006014) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDC123
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:200-4000
• Theoretical MW: 39 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CDC123 Antibody (OTI1F8)

• cell division cycle 123 homolog (S. cerevisiae)
• cell division cycle protein 123 homolog
• chromosome 10 open reading frame 7
• D123C10orf7
• FLJ13863
• HT-1080
• Protein D123
• PZ32

Background

CDC123 is required for S phase entry of the cell cycle

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDC123