CD99 Antibody (3B2/TA8) - BSA Free

Flow Cytometry: CD99 Antibody (3B2/TA8) [NBP3-09116] - Mass cytometry (surface staining) of human peripheral blood cells (after separation using Ficoll-Paque density gradient centrifugation) with anti-human CD99 (3B2/TA8) Eu153. Gated on singlets.

Flow Cytometry: CD99 Antibody (3B2/TA8) [NBP3-09116] - Surface staining pattern of human peripheral blood stained using anti-human CD99 (3B2/TA8) purified antibody (concentration in sample 2 ug/ml) GAM APC.

Flow Cytometry: CD99 Antibody (3B2/TA8) [NBP3-09116] - Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD99 (3B2/TA8) purified antibody (concentration in sample 2 ug/ml) GAM APC.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 3B2/TA8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CD99 Antibody (3B2/TA8) - BSA Free Summary

• Additional Information: Clone 3B2/TA8 was used by HLDA to establish CD designation.
• Immunogen: Human thymocytes
• Specificity: The mouse monoclonal antibody 3B2/TA8 recognizes CD99, an approximately 32 kDa sialoglycoprotein expressed on the surface of many cell types, with particularly strong expression on Ewing's sarcoma and peripheral primitive neuroectodermal tumors. Within the hematopoietic system, CD99 is expressed on virtually all cell types except granulocytes.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD99
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1-4 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD99 Antibody (3B2/TA8) - BSA Free

• 12E7
• CD99 antigenY homolog
• CD99 molecule
• CD99
• E2 antigen
• HBA71
• MIC2 (monoclonal 12E7)
• MIC2
• MIC2Y
• MSK5X
• pilr-1
• PILR-L
• Protein MIC2
• surface antigen MIC2
• T-cell surface glycoprotein E2

Background

CD99 is encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD99