CD99 Antibody (12E7)

Immunohistochemistry-Paraffin: CD99 Antibody (12E7) [NBP2-44694] - Human Ewing's Sarcoma stained with CD99 Monoclonal Antibody (12E7).

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC, ICC/IF
• Clone: 12E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CD99 Antibody (12E7) Summary

• Description: Tissue culture supernatant with 0.05% Azide.
  
  Store at 2 to 8C.
• Additional Information: Clone 12E7 was used by HLDA to establish CD designation.
• Immunogen: Human acute lymphocytic leukemia T-cells
• Localization: Cell surface
• Marker: Ewing's Sarcoma Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD99
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5-10ul/million cells
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:100
• Application Notes: Immunohistochemistry (Formalin-fixed): 1:50-1:100 for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Tissue culture supernatant
• Preservative: 0.05% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for CD99 Antibody (12E7)

• 12E7
• CD99 antigenY homolog
• CD99 molecule
• CD99
• E2 antigen
• HBA71
• MIC2 (monoclonal 12E7)
• MIC2
• MIC2Y
• MSK5X
• pilr-1
• PILR-L
• Protein MIC2
• surface antigen MIC2
• T-cell surface glycoprotein E2

Background

CD99 is encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. Cyclophilin A binds to CD99 and may act as a signaling regulator of CD99. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD99
• Uniprot:
  • P14209()