CD94 Antibody (1032011) [Alexa Fluor™ Plus 405]

• Clone: 1032011
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 405

CD94 Antibody (1032011) [Alexa Fluor™ Plus 405] Summary

• Specificity: Detects human CD94 in direct ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD94 Antibody (1032011) [Alexa Fluor™ Plus 405]

• CD94 antigen
• CD94
• CD94natural killer cells antigen CD94
• Killer cell lectin-like receptor subfamily D member 1
• killer cell lectin-like receptor subfamily D, member 1
• KLRD1
• KP43
• NK cell receptor

Background

CD94 is an approximately 25 kDa type 2 transmembrane protein that plays an important role in regulating natural killer (NK) cell activation (1). Human CD94 consists of a 10 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 148 aa extracellular domain (ECD) with a stem region and one C-type lectin domain (2). Alternative splicing generates additional isoforms that lack either the stem region, the terminal half of the ECD, or the cytoplasmic and transmembrane regions (3). Within the ECD, human CD94 shares 53% and 55% amino acid identity to mouse and rat CD94, respectively. CD94 is expressed at varying cell surface density on NK cells during their differentiation and on a subset of CD8⁺ T cells (4). It associates into disulfide-linked heterodimers with NKG2A/B, C, or E (5-8), and these complexes function as receptors for the nonclassical MHC class I molecule, HLA-E (9, 10). Ligation of CD94-NKG2A or CD94-NKG2C on NK cells triggers inhibitory or activating signals, respectively (11).

1. Chester, C. et al. (2015) Front. Immunol. 6:601.
2. Chang, C. et al. (1995) Eur. J. Immunol. 25:2433.
3. Lieto, L.D. et al. (2006) Genes Immun. 7:36.
4. Yu, J. et al. (2010) Blood 115:274.
5. Phillips, J.H. et al. (1996) Immunity 5:163.
6. Lazetic, S. et al. (1996) J. Immunol. 157:4741.
7. Brooks, A.G. et al. (1997) J. Exp. Med. 185:795.
8. Carretero, M. et al. (1997) Eur. J. Immunol. 27:563.
9. Braud, V.M. et al. (1998) Nature 391:795.
10. Kraemer, T. et al. (2014) J. Immunol. Res. 2014:352160.
11. Houchins, J.P. et al. (1997) J. Immunol. 158:3603.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.