CD82/Kai-1 Antibody (C33)

Images

 
Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of human tonsil (paraffin-embedded sections) with anti-CD82 (C33).
Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Analysis (surface staining) of CD82 on human peripheral blood cells with anti-CD82 (C33) PE.
Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of CD82 on human peripheral blood cells with anti-CD82 (C33) APC.
Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Flow Cytometry: [PerCP] [NBP2-21792PCP] - A cell surface (A) and intracellular stain (B) was performed on Daudi cells with CD82/Kai-1 (C33) antibody ...read more
Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of CD82 on human peripheral blood cells with anti-CD82 (C33) FITC.
Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Analysis (surface staining) of CD82 on human peripheral blood cells with anti-CD82 (C33) biotin, streptavidin/PE.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready
Clone
C33
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CD82/Kai-1 Antibody (C33) Summary

Immunogen
C91/PL (human HTLV-1+ T cell line).
Specificity
The antibody C33 reacts with CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CD82
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Flow Cytometry 1:50-1:250
  • Immunocytochemistry/Immunofluorescence 1:50-1:300
  • Immunohistochemistry 1:50-1:300
  • Immunohistochemistry-Paraffin 1:50-1:300
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
Application Notes
In human MOLT-4 T-cell line the antibody C33 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines. This antibody is CyTOF ready.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for CD82/Kai-1 Antibody (C33)

  • CD82 antigenC33
  • CD82 molecule
  • CD82
  • IA4
  • IA4C33 antigen
  • Inducible membrane protein R2
  • KAI1
  • KAI1GR15
  • kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyteantigen, antigen detected by monoclonal and IA4))
  • Metastasis suppressor Kangai-1
  • R2
  • SAR2
  • SAR2tetraspanin-27
  • ST6
  • ST6tspan-27
  • Suppressor of tumorigenicity 6 protein
  • Tetraspanin-27
  • TSPAN27
  • Tspan-27
  • TSPAN274F9

Background

CD82 (KAI1), a member of the tetraspanin family, forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules. These complexes influence adhesion, morphology, activation, proliferation and differentiation of B, T and other cells. CD82 regulates cytoskeleton rearrangement and may participate in the turnover of the tetraspanin complex members. Besides in the plasma membrane, CD82 is localized also in endosome/lysosome compartments. Tumour-suppressive roles of CD82 have been demonstrated.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD82/Kai-1 Antibody (NBP2-21792) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-21792AF350
Alexa Fluor 405 NBP2-21792AF405
Alexa Fluor 488 NBP2-21792AF488
Alexa Fluor 532 NBP2-21792AF532
Alexa Fluor 594 NBP2-21792AF594
Alexa Fluor 647 NBP2-21792AF647
Alexa Fluor 700 NBP2-21792AF700
Alexa Fluor 750 NBP2-21792AF750
Allophycocyanin NBP2-21792APC
Allophycocyanin/Cy7 NBP2-21792APCCY7
DyLight 350 NBP2-21792UV
DyLight 405 NBP2-21792V
DyLight 488 NBP2-21792G
DyLight 550 NBP2-21792R
DyLight 594 NBP2-21792DL594
DyLight 650 NBP2-21792C
DyLight 680 NBP2-21792FR
DyLight 755 NBP2-21792IR
Janelia Fluor 549 NBP2-21792JF549
Janelia Fluor 646 NBP2-21792JF646
PE NBP2-21792PE
PE/Atto594 NBP2-21792PEATT594
PE/Cy5.5 NBP2-21792PECY55
PE/Cy7 NBP2-21792PECY7
PerCP NBP2-21792PCP

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