![Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of human tonsil (paraffin-embedded sections) with anti-CD82 (C33).](http://images.novusbio.com/fullsize2/CD82-Kai-1-Antibody-(C33)-Immunohistochemistry-Paraffin-NBP2-21792-img0002.jpg "Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of human tonsil (paraffin-embedded sections) with anti-CD82 (C33).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IHC-P, IP, CyTOF-ready |
| Clone | C33 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | C91/PL (human HTLV-1+ T cell line). |
| Specificity | The antibody C33 reacts with CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CD82 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | In human MOLT-4 T-cell line the antibody C33 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines. This antibody is CyTOF ready. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.4) |
| Preservative | 15mM Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
![Western Blot: RRM1 Antibody [NBP2-20231] - A. 30 ug NIH-3T3 whole cell lysate/extract B. 30 ug Raw264. 7 whole cell lysate/extract C. 30 ug C2C12 whole cell lysate/extract5 % SDS-PAGE RRM1 antibody dilution: 1:1000.](http://images.novusbio.com/images2/RRM1-Antibody-Western-Blot-NBP2-20231-img0001.jpg)
![Western Blot: RRM1 Antibody [NBP2-20231] - Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/images2/RRM1-Antibody-Western-Blot-NBP2-20231-img0002.jpg)
![Immunohistochemistry-Paraffin: RRM1 Antibody [NBP2-20231] - Human colon carcinoma, using RRM1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/images2/RRM1-Antibody-Immunohistochemistry-Paraffin-NBP2-20231-img0003.jpg)
![Flow Cytometry: CD1b Antibody (CC20) [NB100-65315] - Figure A. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody and RPE conjugated Mouse IgG2a isotype control. Figure B. FITC conjugated Mouse anti Bovine MHC Class II Monomorphic Antibody and RPE conjugated Mouse anti Bovine CD1w2 Antibody. All experiments performed on bovine thymocytes in the presence of 10% bovine serum and gated on live, single cell thymocytes.](http://images.novusbio.com/images2/CD1b-Antibody-(CC20)-Flow-Cytometry-NB100-65315-img0001.jpg)
![Flow Cytometry: CD1b Antibody (CC20) [NB100-65315] - Figure A. FITC conjugated Mouse anti Bovine CD4 Antibody and Mouse IgG2a isotype control conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody. Figure B. FITC conjugated Mouse anti Bovine CD4 Antibody and Mouse anti Bovine CD1w2 Antibody conjugated with Rabbit F(ab')2 anti Mouse IgG:RPE Antibody.](http://images.novusbio.com/images2/CD1b-Antibody-(CC20)-Flow-Cytometry-NB100-65315-img0002.jpg)
![Flow Cytometry: CD1b Antibody (CC20) [NB100-65315] - Figure A. FITC conjugated Mouse anti Bovine CD4 Antibody and RPE conjugated Mouse IgG2a isotype control. Figure B. FITC conjugated Mouse anti Bovine CD4 Antibody and RPE conjugated Mouse anti Bovine CD1w2 Antibody.](http://images.novusbio.com/images2/CD1b-Antibody-(CC20)-Flow-Cytometry-NB100-65315-img0003.jpg)
![Protein Array: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HLA DQ/DR/DP Antibody (HLA-Pan/2967R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/images2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Protein-Array-NBP2-79843-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Immunofluorescence staining of Raji cells using HLA DQ/DR/DP Antibody (HLA-Pan/2967R) followed by goat anti-rabbit IgG-CF488 (Green). Nuclei stained with RedDot (Red).](http://images.novusbio.com/images2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-79843-img0002.jpg)
![Western Blot: HLA DQ/DR/DP Antibody (HLA-Pan/2967R) - Azide and BSA Free [NBP2-79843] - Western Blot Analysis of Ramos cell lysate using HLA DQ/DR/DP Antibody (HLA-Pan/2967R).](http://images.novusbio.com/images2/HLA-DQ-DR-DP-Antibody-(HLA-Pan-2967R)---Azide-and-BSA-Free-Western-Blot-NBP2-79843-img0003.jpg)
![Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Flow-Cytometry-NB100-524-img0014.jpg)
![Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Flow-Cytometry-NB100-524-img0015.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/images2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) APC.](http://images.novusbio.com/images2/CD9-Antibody-(MEM-61)-Flow-Cytometry-NB500-327-img0006.jpg)
![Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) PE.](http://images.novusbio.com/images2/CD9-Antibody-(MEM-61)-Flow-Cytometry-NB500-327-img0007.jpg)
![Flow Cytometry: CD9 Antibody (MEM-61) [NB500-327] - Staining of human peripheral blood with anti-CD9 (MEM-61) Alexa Fluor® 647.](http://images.novusbio.com/images2/CD9-Antibody-(MEM-61)-Flow-Cytometry-NB500-327-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-CD63 [H5C6] conjugated to Alexa Fluor 488 [NBP2-42225AF488] at 10ug/mL for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/CD63-Antibody-(H5C6)-Immunocytochemistry-Immunofluorescence-NBP2-42225-img0008.jpg)
![Flow (Intracellular): CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on HepG2 cells with CD63 Antibody (H5C6) NBP2-42225AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/images2/CD63-Antibody-(H5C6)-Flow-(Intracellular)-NBP2-42225-img0009.jpg)
![Western Blot: CD63 Antibody (H5C6) [NBP2-42225] - The same samples and volumes were run under reducing and non-reducing conditions. All procedures were performed in parallel for both conditions. L- ladder +- exosomes. Image visualized with HRP linked to secondary antibody. Western blot image submitted by a verified customer review.](http://images.novusbio.com/images2/CD63-Antibody-(H5C6)-Western-Blot-NBP2-42225-img0007.jpg)
![Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat Cells with CD81 Antibody (1D6) NB100-65805B and a matched isotype control. Both antibodies were conjugated to biotin. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).](http://images.novusbio.com/images2/CD81-Antibody-(1D6)-Flow-Cytometry-NB100-65805-img0013.jpg)
![Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 Antibody [1D6] NB100-65805AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/images2/CD81-Antibody-(1D6)-Flow-Cytometry-NB100-65805-img0014.jpg)
![Western Blot: CD81 Antibody (1D6) [NB100-65805] - Isolation and characterization of exosomes from cell culture supernatant and human blood. Immunoblot of exosomal markers CD81 (~30 kDa), CD63 (~55 kDa), and LAMP2B (~50 kDa) in exosomes (5 ug lysates) isolated from the serum of breast cancer patients and healthy control. Grp94 (~100 kDa) and beta-actin (~42 kDa) serve as a negative control and a loading control, respectively. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep36502), licensed under a CC-BY licence.](http://images.novusbio.com/images2/CD81-Antibody-(1D6)-Western-Blot-NB100-65805-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Adenine Nucleotide Translocator 2 Antibody [NBP2-92630] - Analysis of HeLa cells using Adenine Nucleotide Translocator 2 . Blue: DAPI for nuclear staining.](http://images.novusbio.com/images2/Adenine-Nucleotide-Translocator-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92630-img0001.jpg)
![Immunohistochemistry-Paraffin: Adenine Nucleotide Translocator 2 Antibody [NBP2-92630] - Paraffin-embedded mouse heart using Adenine Nucleotide Translocator 2 .](http://images.novusbio.com/images2/Adenine-Nucleotide-Translocator-2-Antibody-Immunohistochemistry-Paraffin-NBP2-92630-img0002.jpg)
![Western Blot: Adenine Nucleotide Translocator 2 Antibody [NBP2-92630] - Analysis of extracts of various cell lines, using Adenine Nucleotide Translocator 2 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.](http://images.novusbio.com/images2/Adenine-Nucleotide-Translocator-2-Antibody-Western-Blot-NBP2-92630-img0004.jpg)
![Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Flow-Cytometry-NB200-103-img0006.jpg)
![Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Flow-Cytometry-NB200-103-img0007.jpg)
![Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.](http://images.novusbio.com/images2/p53-Antibody-(PAb-240)-Flow-(Intracellular)-NB200-103-img0003.jpg)
Secondary Antibodies |
Isotype Controls |
Diseases for CD82/Kai-1 Antibody (NBP2-21792)Discover more about diseases related to CD82/Kai-1 Antibody (NBP2-21792).
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![Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Analysis (surface staining) of CD82 on human peripheral blood cells with anti-CD82 (C33) PE.](http://images.novusbio.com/fullsize2/CD82-Kai-1-Antibody-(C33)-Flow-Cytometry-NBP2-21792-img0007.jpg "Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Analysis (surface staining) of CD82 on human peripheral blood cells with anti-CD82 (C33) PE.")
![Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of CD82 on human peripheral blood cells with anti-CD82 (C33) APC.](http://images.novusbio.com/fullsize2/CD82-Kai-1-Antibody-(C33)-Flow-Cytometry-NBP2-21792-img0001.jpg "Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of CD82 on human peripheral blood cells with anti-CD82 (C33) APC.")
![Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Flow Cytometry: [PerCP] [NBP2-21792PCP] - A cell surface (A) and intracellular stain (B) was performed on Daudi cells with CD82/Kai-1 (C33) antibody NBP2-21792PCP along with a matched isotype control NB600-986PCP. The cell surface stain was performed in an antibody dilution of 5 ug/mL and incubated at RT for 20 minutes. Prior to intracellular staining, the cells were fixed with 4% PFA and then permeablized with 0.1 % saponin. The cells were then incubated in an antibody dilution of 5 ug/mL at RT for 30 minutes. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex. Image using the PerCP form of this antibody.](http://images.novusbio.com/fullsize2/CD82-Kai-1-Antibody-(C33)-Flow-Cytometry-NBP2-21792-img0003.jpg "Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Flow Cytometry: [PerCP] [NBP2-21792PCP] - A cell surface (A) and intracellular stain (B) was performed on Daudi cells with CD82/Kai-1 (C33) antibody NBP2-21792PCP along with a matched isotype control NB600-986PCP. The cell surface stain was performed in an antibody dilution of 5 ug/mL and incubated at RT for 20 minutes. Prior to intracellular staining, the cells were fixed with 4% PFA and then permeablized with 0.1 % saponin. The cells were then incubated in an antibody dilution of 5 ug/mL at RT for 30 minutes. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex. Image using the PerCP form of this antibody.")
![Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of CD82 on human peripheral blood cells with anti-CD82 (C33) FITC.](http://images.novusbio.com/fullsize2/CD82-Kai-1-Antibody-(C33)-Flow-Cytometry-NBP2-21792-img0005.jpg "Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of CD82 on human peripheral blood cells with anti-CD82 (C33) FITC.")
![Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Analysis (surface staining) of CD82 on human peripheral blood cells with anti-CD82 (C33) biotin, streptavidin/PE.](http://images.novusbio.com/fullsize2/CD82-Kai-1-Antibody-(C33)-Flow-Cytometry-NBP2-21792-img0006.jpg "Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Analysis (surface staining) of CD82 on human peripheral blood cells with anti-CD82 (C33) biotin, streptavidin/PE.")
![Western Blot: CDCA7L Antibody (OTI3B5) [NBP2-46198] - Detection of CDCA7L by western blot. Samples: Whole cell lysate from prostate cancer; 40 ug. Antibodies:CDCA7L Antibody [NBP2-46198] at dilutions 1:500, 1:1000, 1:2000. Detection: Chemiluminescence with exposure of 5 seconds. Image from verified customer review.](http://images.novusbio.com/images2/CDCA7L-Antibody-(OTI3B5)-Western-Blot-NBP2-46198-img0010.jpg)
![Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/CDCA7L-Antibody-(3B5)-Immunohistochemistry-NBP2-46198-img0001.jpg)
![Immunohistochemistry: CDCA7L Antibody (3B5) [NBP2-46198] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/images2/CDCA7L-Antibody-(3B5)-Immunohistochemistry-NBP2-46198-img0002.jpg)
![Flow Cytometry: CD1a Antibody (NA1/34-HLK) [NB100-64852] - Analysis using the FITC conjugate of NB100-64852. Staining of MOLT 4 cells.](http://images.novusbio.com/images2/CD1a-Antibody-(NA1-34-HLK)-Flow-Cytometry-NB100-64852-img0005.jpg)
![Flow Cytometry: CD1a Antibody (NA1/34-HLK) [NB100-64852] - Analysis using the Biotin conjugate of NB100-64852. Staining of MOLT 4 cells with CD1a antibody.](http://images.novusbio.com/images2/CD1a-Antibody-(NA1-34-HLK)-Flow-Cytometry-NB100-64852-img0004.jpg)
![Immunohistochemistry-Frozen: CD1a Antibody (NA1/34-HLK) [NB100-64852] - mmunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD1a antibody followed by HISTAR detection system. Low power](http://images.novusbio.com/images2/CD1a-Antibody-(NA1-34-HLK)-Immunohistochemistry-Frozen-NB100-64852-img0006.jpg)
