CD81 Antibody (454729) [Unconjugated]

CD81 was detected in immersion fixed Jurkat human acute T cell leukemia cell line (left panel; positive staining) and HepG2 human hepatocellular carcinoma cell line (right panel; negative staining) using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # MAB46151) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CD81 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD81 Monoclonal Antibody (Catalog # MAB46151) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC, ICC/IF
• Clone: 454729
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

CD81 Antibody (454729) [Unconjugated] Summary

• Immunogen: HEK293 human embryonic kidney cell line transfected with human CD81
  Accession # P60033
• Specificity: Detects human CD81 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD81 Antibody (454729) [Unconjugated]

• CD81 antigen (target of antiproliferative 1)
• CD81 antigen
• CD81 molecule
• CD81
• CVID6
• TAPA1
• TAPA-1
• Target of the antiproliferative 1
• Tetraspanin-28
• TSPAN28
• Tspan-28
• tspan-28,26 kDa cell surface protein TAPA-1
• TSPAN28S5.7

Background

CD81, also known as TAPA-1 and TM4SF4, is a widely expressed protein in the tetraspanin family. CD81 is a multifunctional protein that interacts with a variety of other molecules, including tetraspanins, and is important for organization of the plasma membrane into microdomains. CD81 facilitates B cell and T cell activation and is an integrin-binding adhesion molecule. CD81 expression on lymphocytes is altered during infection by hepatitis C virus or HIV-1 and contributes to the pathogenicity of those viruses. Human CD81 shares 92%‑93% aa sequence identity with mouse and rat CD81.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P60033()