CD8 beta Antibody (EP42) [FITC]

Flow Cytometry: CD8 beta Antibody (EP42) [FITC] [NBP1-28301] - Chicken IEL were double stained with mouse antichicken CD8beta-FITC and mouse anti-chicken CD8beta-RPE. Small lymphocytes then were gated and analyzed on a FACScan (Trademark) flow cytometer (BDIS, San Jose, CA).

• Reactivity: Ch
• Applications: Flow
• Clone: EP42
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC

CD8 beta Antibody (EP42) [FITC] Summary

• Immunogen: Chicken splenocytes
• Specificity: beta chain (Mr 34 kDa) of chicken CD8
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD8B
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Reactivity Notes

Chicken

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for CD8 beta Antibody (EP42) [FITC]

• CD8 antigen, beta polypeptide 1 (p37)
• CD8 beta
• CD8b antigen
• CD8b molecule
• CD8b
• CD8B1
• CD8B1P37
• Leu2
• LY3
• LYT3
• MGC119115
• P37
• T lymphocyte surface glycoprotein beta chain
• T-cell surface glycoprotein CD8 beta chain

Background

In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain. (1) While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8alpha- and CD8beta-chains, a relatively large proportion of the CD8+ TCR-gamma delta cells in the spleens of embryos and young chicks express only the alpha-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR-gamma delta CD8alpha beta cells in the chicken that is not found in rodents. (3) Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells and 50% of spleen cells, but <1% of cells in the bursa and bone marrow. (1,2) MAb EP42 recognizes the CD8beta chain. (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD8B