CD8 beta Antibody (EP42)

Images

 
Flow Cytometry: CD8 beta Antibody (EP42) [NBP1-28300] - Analysis using the FITC conjugate of NBP1-28300. Double staining of Chicken IEL with mouse antichicken CD8beta-FITC and mouse anti-chicken CD8beta-RPE. Small ...read more
Flow Cytometry: CD8 beta Antibody (EP42) [NBP1-28300] - Analysis using the Biotin conjugate of NBP1-28300. Multiple staining of peripheral blood mononuclear cells.

Product Details

Summary
Reactivity ChSpecies Glossary
Applications Flow, IHC, IHC-Fr, IP
Clone
EP42
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

CD8 beta Antibody (EP42) Summary

Immunogen
Chicken splenocytes
Specificity
beta chain (Mr 34 kDa) of chicken CD8
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD8B
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Chicken

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD8 beta Antibody (EP42)

  • CD8 antigen, beta polypeptide 1 (p37)
  • CD8 beta
  • CD8b antigen
  • CD8b molecule
  • CD8b
  • CD8B1
  • CD8B1P37
  • Leu2
  • LY3
  • LYT3
  • MGC119115
  • P37
  • T lymphocyte surface glycoprotein beta chain
  • T-cell surface glycoprotein CD8 beta chain

Background

In the chicken, the CD8 molecule is present in two forms: (i) a homodimer of two alpha chains, and (ii) a heterodimer of an alpha chain and a beta chain. (1) While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8alpha- and CD8beta-chains, a relatively large proportion of the CD8+ TCR-gamma delta cells in the spleens of embryos and young chicks express only the alpha-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved, but there is a population of TCR-gamma delta CD8alpha beta cells in the chicken that is not found in rodents. (3) Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells and 50% of spleen cells, but <1% of cells in the bursa and bone marrow. (1,2) MAb EP42 recognizes the CD8beta chain. (1)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-28300AF405
Alexa Fluor 488 Labeled NBP1-28300AF488
Alexa Fluor 647 Labeled NBP1-28300AF647
Alexa Fluor 700 Labeled NBP1-28300AF700
Allophycocyanin Labeled NBP1-28300APC
DyLight 350 Labeled NBP1-28300UV
DyLight 405 Labeled NBP1-28300V
DyLight 488 Labeled NBP1-28300G
DyLight 680 Labeled NBP1-28300FR
DyLight 755 Labeled NBP1-28300IR
PE Labeled NBP1-28300PE
PerCP Labeled NBP1-28300PCP

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Bioinformatics

Gene Symbol CD8B
Entrez