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CD69 Antibody

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Western Blot: CD69 Antibody [NBP1-68485] - Analysis of extracts from HuvEc cells, using CD69 Antibody. The lane on the right is treated with the synthesized peptide.
Western Blot: CD69 Antibody [NBP1-68485] - Analysis of extracts from HuvEc cells, using CD69 Antibody. The lane on the right is treated with the synthesized peptide.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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CD69 Antibody Summary

Immunogen
The antiserum was produced against synthesized peptide derived from human CD69. The immunogen region is: Internal.
Specificity
CD69 Antibody detects endogenous levels of total CD69 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD69
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:4000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+, pH 7.4), 0.15M NaCl and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD69 Antibody

  • activation inducer molecule (AIM/CD69)
  • Activation inducer molecule
  • AIM
  • BL-AC/P26
  • CD69 antigen (p60, early T-cell activation antigen)
  • CD69 antigen
  • CD69 molecule
  • CD69
  • CLEC2CC-type lectin domain family 2 member C
  • C-type lectin domain family 2, member C
  • EA1
  • EA-1
  • early activation antigen CD69
  • early lymphocyte activation antigen
  • Early T-cell activation antigen p60
  • GP32/28
  • Leu23
  • Leukocyte surface antigen Leu-23
  • MLR-3
  • p60

Background

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3 and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection. The H1.2F3 antibody has been reported to augment T cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human CTLA-4. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CTLA‑4 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
NS0 mouse myeloma cell line co-transfected with human CTLA-4 and eGFP was stained with either (A) Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) or (B) Normal Goat IgG Control (Catalog # AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
CTLA‑4 was detected in immersion fixed human peripheral blood mononuclear cells treated with treated with PMA and calcium ionomycin using Goat Anti-Human CTLA‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-386-PB) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.
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Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human lung shows high expression.
Immunohistochemistry-Paraffin: HLA DQA1 Antibody [NBP1-84550] - Staining of human tonsil shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
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Applications: WB, IHC, IHC-P
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202-IL/CF
Recombinant Human IL-2 (Catalog # 202-IL/CF) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
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Flow Cytometry: CD3 Antibody (OKT3) - Azide Free [NBP2-24867] - Surface staining of human PBMC with CD3 antibody (red) grown in serum-free media and isotype control (green) at 0.25 ug/10^6 cells.
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(37407) [Unconjugated
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L-Selectin/CD62L
Antibody (DREG56)
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Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Analysis using the DyLight 405 conjugate of NBP1-42795. Staining of L-Selectin in human PBMCs using anti-L-Selectin antibody. Image from verified customer review.
Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Analysis using the FITC conjugate of NBP1-42795. Staining of normal human peripheral blood cells with Anti-Human CD4 APC and Mouse IgG1 K Isotype Control FITC (left) or Anti-Human CD62L (L-Selectin) FITC (right). Cells in the lymphocyte gate were used for analysis.
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(BU63)
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Immunohistochemistry-Paraffin: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Formalin-fixed, paraffin-embedded esophagus tumor tissue stained with CD86 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen. Note strong staining of differentiated squamous cells. TMA was used for this test.
Flow Cytometry: B7-2/CD86 Antibody (BU63) [NBP2-25208] - Surface staining of CD86 on 10^6 PBMC using 0.25 ug of CD86 antibody.
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Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Confocal analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) [NBP1-47386] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human breast carcinoma tissues using CD44 antibody (clone 8E2F3). The signal was developed using DAB based detection and the sections were processed for counterstaining with hematoxylin. The antibody generated mainly a membrane staining representative of CD44 protein.
Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - Flow cytometric analysis of Hela cells using anti-CD44 mAb (right) and negative control (left).
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CD69 Antibody
NBP1-68485
CD69 Antibody
Species: Hu, Mu, Rt
Applications: WB, ELISA

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Bioinformatics

Gene Symbol CD69
Entrez
Uniprot