CD69 Antibody (H1.2F3)

Images

 
Flow Cytometry: CD69 Antibody (H1.2F3) [NBP1-28011] - PMA and Ionomycin stimulated BALB/c mouse splenocytes were stained with Hamster Anti-Mouse CD69-PACBLU.
Flow Cytometry: CD69 Antibody (H1.2F3) [NBP1-28011] - Analysis using the FITC conjugate of NBP1-28011. Less than or equal to 1 ug/10^6 Fresh BALB/c spleen cells were activated by incubation for 20 hours with PMA + ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IHC-Fr, In vitro, IP, BA
Clone
H1.2F3
Clonality
Monoclonal
Host
Armenian Hamster
Conjugate
Unconjugated
Concentration
0.5 mg/ml

CD69 Antibody (H1.2F3) Summary

Immunogen
Mouse dendritic epidermal cell line Y245
Specificity
Mouse CD69/Very Early Activation Antigen (VEA), Mr 85 kDa (unreduced)
Isotype
IgG1
Clonality
Monoclonal
Host
Armenian Hamster
Gene
CD69
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • In vitro assay
  • Bioactivity
Application Notes
Flow Cytometry, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen and In vitro assay

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for CD69 Antibody (H1.2F3)

  • activation inducer molecule (AIM/CD69)
  • Activation inducer molecule
  • AIM
  • BL-AC/P26
  • CD69 antigen (p60, early T-cell activation antigen)
  • CD69 antigen
  • CD69 molecule
  • CD69
  • CLEC2CC-type lectin domain family 2 member C
  • C-type lectin domain family 2, member C
  • EA1
  • EA-1
  • early activation antigen CD69
  • early lymphocyte activation antigen
  • Early T-cell activation antigen p60
  • GP32/28
  • Leu23
  • Leukocyte surface antigen Leu-23
  • MLR-3
  • p60

Background

CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa. (1,2) It is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. (3) CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils. (1-4) Although not detectable on resting lymphocytes, its expression is rapidly (within 2 hours) upregulated upon activation of T, B and NK cells, and neutrophils. (4) Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells. (4,5) MAb H1.2F3 augments PMA-induced T-cell proliferation,1,6 and induces redirected lysis of Fc receptor-bearing target cells by NK cells. (7)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-28011AF405
Alexa Fluor 488 Labeled NBP1-28011AF488
Alexa Fluor 647 Labeled NBP1-28011AF647
Alexa Fluor 700 Labeled NBP1-28011AF700
Allophycocyanin Labeled NBP1-28011APC
DyLight 350 Labeled NBP1-28011UV
DyLight 405 Labeled NBP1-28011V
DyLight 488 Labeled NBP1-28011G
DyLight 680 Labeled NBP1-28011FR
DyLight 755 Labeled NBP1-28011IR
FITC Labeled NBP1-28012
PE Labeled NBP1-28011PE
PerCP Labeled NBP1-28011PCP

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Bioinformatics

Gene Symbol CD69
Entrez