CD69 Antibody (298614) [Phycoerythrin]

Human peripheral blood lymphocytes either untreated (open histogram) or treated with PHA (filled histogram) were stained with Mouse Anti-Human CD69 PE-conjugated Monoclonal Antibody (Catalog # FAB23591P) or isotype control antibody (IC003P, data not shown). View our protocol for Staining Membrane-associated Proteins.

PBMCs either treated with 5 ug/mL PHA for 2 days (A) or (B) left untreated (naive) were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and Mouse Anti-Human CD69 PE‑conjugated Monoclonal Antibody (Catalog # FAB23591P). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 298614
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

CD69 Antibody (298614) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD69
  Gly64-Lys199
  Accession # Q07108
• Specificity: Detects human CD69 in Western blots. In Western blots, no cross-reactivity with mouse CD69 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD69
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD69 Antibody (298614) [Phycoerythrin]

• activation inducer molecule (AIM/CD69)
• Activation inducer molecule
• AIM
• BL-AC/P26
• CD69 antigen (p60, early T-cell activation antigen)
• CD69 antigen
• CD69 molecule
• CD69
• CLEC2CC-type lectin domain family 2 member C
• C-type lectin domain family 2, member C
• EA1
• EA-1
• early activation antigen CD69
• early lymphocyte activation antigen
• Early T-cell activation antigen p60
• GP32/28
• Leu23
• Leukocyte surface antigen Leu-23
• MLR-3
• p60

Background

CD69, also known as Leu 23, AIM, EA-1, and MLR-3, is a type II transmembrane glycoprotein and is expressed on activated T cells, B cells, NK cells, neutrophils and eosinophils, Langerhans cells, and platelets.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD69 Antibody (FAB23591P)(5)

Publications using FAB23591P

• JH Cha, LC Chan, YN Wang, YY Chu, CH Wang, HH Lee, W Xia, WC Shyu, SP Liu, J Yao, CW Chang, FR Cheng, J Liu, SO Lim, JL Hsu, WH Yang, GN Hortobagyi, C Lin, L Yang, D Yu, LB Jeng, MC Hung Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer The Journal of Biological Chemistry, 2022-03-10;0(0):101817. 2022-03-10 [PMID: 35278434] (Flow Cytometry, Human)
• Qingqu Guo, Yiming Chu, Hongbo Li, Dike Shi, Lele Lin, Weifeng Lan et al. Dickkopf-related protein 3 alters aerobic glycolysis in pancreatic cancer BxPC-3 cells, promoting CD4+ T-cell activation and function European Journal of Medical Research 12/1/2021 [PMID: 34391478]
• Wenbin Ji, Jin Chen, Yuche Mi, Guiliang Wang, Xinjiang Xu, Weizheng Wang Role of natural killer cells in liver transplantation treatment of liver cancer Experimental and Therapeutic Medicine 9/1/2017 [PMID: 28962171]
• Giuliani M, Fleury M, Vernochet A, Ketroussi F, Clay D, Azzarone B, Lataillade JJ, Durrbach A Long-lasting inhibitory effects of fetal liver mesenchymal stem cells on T-lymphocyte proliferation. 2011-05-19;6(5):e19988. 2011-05-19 [PMID: 21625521] (Flow Cytometry, Human)
• Ni Z, Walcheck B Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation. Clin. Immunol., 2009-08-08;133(2):257-64. 2009-08-08 [PMID: 19665434] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CD69
• Uniprot:
  • Q07108()