CD68/SR-D1 Antibody (298807) [Unconjugated]

CD68/SR‑D1 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human CD68/SR‑D1 Monoclonal Antibody (Catalog # MAB20401) at 0.1 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface of Kupffer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 298807
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD68/SR-D1 Antibody (298807) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD68/SR‑D1
  Asn22-Ile320
  Accession # AAB25811
• Specificity: Detects human CD68/SR‑D1 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD68
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.1-10 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD68/SR-D1 Antibody (298807) [Unconjugated]

• CD_antigen: CD68
• CD68 antigen
• CD68 molecule
• CD68
• DKFZp686M18236
• gp110
• LAMP4
• macrophage antigen CD68
• Macrosialin
• SCARD1
• scavenger receptor class D, member 1
• SRD1
• SR-D1

Background

CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein that belongs to the LAMP family of molecules. CD68 is expressed on monocytes and macrophages and serves as a scavenger receptor for oxidized LDL.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD68/SR-D1 Antibody (MAB20401)(18)

Publications using MAB20401

• Belgiovine, C;Digifico, E;Erreni, M;Putignano, AR;Mannarino, L;Valentino, S;Grizzi, F;Pasqualini, F;Recordati, C;Bertola, L;Zucali, P;Pistillo, D;Paleari, V;Mantovani, A;D'Incalci, M;Marchesi, F;Allavena, P; Malignant mesothelioma-associated inflammatory microenvironment promotes tumor progression via GPNMB Journal of translational medicine 2025-04-18 [PMID: 40251684] (Immunohistochemistry, Human)
• Ling, J;You, S;Chen, W;Yang, X;Xv, Y;Zhu, B; Galectin-9 as a new biomarker of acute-on-chronic liver failure Scientific reports 2024-09-27 [PMID: 39333198] (Immunohistochemistry, Human)
• Kirsten, N;Ohmes, J;Mikkelsen, MD;Nguyen, TT;Blümel, M;Wang, F;Tasdemir, D;Seekamp, A;Meyer, AS;Fuchs, S; Impact of Enzymatically Extracted High Molecular Weight Fucoidan on Lipopolysaccharide-Induced Endothelial Activation and Leukocyte Adhesion Marine drugs 2023-05-31 [PMID: 37367664] (ICC, Human)
• Gillan, JL;Chokshi, M;Hardisty, GR;Clohisey Hendry, S;Prasca-Chamorro, D;Robinson, NJ;Lasota, B;Clark, R;Murphy, L;Whyte, MKB;Baillie, JK;Davidson, DJ;Bao, G;Gray, RD; CAGE sequencing reveals CFTR-dependent dysregulation of type I IFN signaling in activated cystic fibrosis macrophages Science advances 2023-05-26 [PMID: 37235648] (ICC, Human)
• Emilia Barreto-Duran, Artur Szczepański, Adrianna Gałuszka-Bulaga, Marcin Surmiak, Maciej Siedlar, Marek Sanak et al. The interplay between the airway epithelium and tissue macrophages during the SARS-CoV-2 infection Frontiers in Immunology 2022-10-06 [PMID: 36275746]
• F Real, A Zhu, B Huang, A Belmellat, A Sennepin, T Vogl, C Ransy, M Revol, R Arrigucci, A Lombès, J Roth, ML Gennaro, F Bouillaud, S Cristofari, M Bomsel S100A8-mediated metabolic adaptation controls HIV-1 persistence in macrophages in vivo Nature Communications, 2022-10-11;13(1):5956. 2022-10-11 [PMID: 36220814] (ICC, Human)
• RM Gopalakris, M Aid, NB Mercado, C Davis, S Malik, E Geiger, V Varner, R Jones, SE Bosinger, C Piedra-Mor, AJ Martinot, DH Barouch, RK Reeves, CS Tan Increased IL-6 expression precedes reliable viral detection in the rhesus macaque brain during acute SIV infection JCI Insight, 2021-10-22;6(20):. 2021-10-22 [PMID: 34676832] (IHC, Primate)
• Z Feng, J Zhou, Y Liu, R Xia, Q Li, L Yan, Q Chen, X Chen, Y Jiang, G Chao, M Wang, G Zhou, Y Zhang, Y Wang, H Xia Epithelium- and endothelium-derived exosomes regulate the alveolar macrophages by targeting RGS1 mediated calcium signaling-dependent immune response Cell Death and Differentiation, 2021-03-22;0(0):. 2021-03-22 [PMID: 33753901] (ELISA Control, Human, Mouse)
• Liping Qi, Kui Wu, Shutian Shi, Qingwei Ji, Huangtai Miao, Que Bin Thrombospondin‑2 is upregulated in patients with aortic dissection and enhances angiotensin II‑induced smooth muscle cell apoptosis Experimental and Therapeutic Medicine 2020-10-06 [PMID: 33093888] (Immunohistochemistry, Human)
• P Zhao, B Wang, Z Zhang, W Zhang, Y Liu Response gene to complement 32 expression in macrophages augments paracrine stimulation-mediated colon cancer progression Cell Death Dis, 2019-10-10;10(10):776. 2019-10-10 [PMID: 31601783] (IHC-P, Human)
• JN Ahmad, J Holubova, O Benada, O Kofronova, L Stehlik, M Vasakova, P Sebo Bordetella Adenylate Cyclase Toxin Inhibits Monocyte-to-Macrophage Transition and Dedifferentiates Human Alveolar Macrophages into Monocyte-like Cells MBio, 2019-09-24;10(5):. 2019-09-24 [PMID: 31551332] (Western Blot, Human)
• Kyoungrae Kim, Shihuan Kuang, Qifan Song, Timothy P. Gavin, Bruno T. Roseguini Impact of heat therapy on recovery after eccentric exercise in humans Journal of Applied Physiology 2019-04-01 [PMID: 30605396]
• BJ Silva, MG Barbosa, PR Andrade, H Ferreira, JA Nery, S C“rte-Real, GM da Silva, PS Rosa, M Fabri, EN Sarno, RO Pinheiro Autophagy Is an Innate Mechanism Associated with Leprosy Polarization PLoS Pathog, 2017-01-05;13(1):e1006103. 2017-01-05 [PMID: 28056107] (ICC, Human)
• Liu T, Zhang X, Zhang J, Liang Z, Cai W, Huang M, Yan C, Zhu Z, Han Y Association between PON1 rs662 polymorphism and coronary artery disease. Eur J Clin Nutr, 2014-06-11;68(9):1029-35. 2014-06-11 [PMID: 24918121] (IHC-P, Human)
• Shan M, Cheng HF, Song LZ Lung myeloid dendritic cells coordinately induce TH1 and TH17 responses in human emphysema. Sci Transl Med, 2009-10-28;1(4):4ra10. 2009-10-28 [PMID: 20368170] (ICC, IHC-Fr, Human)
• Lu TC, Wang ZH, Feng X, Chuang PY, Fang W, Shen Y, Levy DE, Xiong H, Chen N, He JC Knockdown of Stat3 activity in vivo prevents diabetic glomerulopathy. Kidney Int., 2009-04-08;76(1):63-71. 2009-04-08 [PMID: 19357722] (IHC-P, Mouse)
• Lumeng CN, Deyoung SM, Bodzin JL, Saltiel AR Increased inflammatory properties of adipose tissue macrophages recruited during diet-induced obesity. Diabetes, 2007-01-01;56(1):16-23. 2007-01-01 [PMID: 17192460] (ICC, Mouse)
• Yang SY, Nasser S, Markel DC, Robbins PD, Wooley PH Human periprosthetic tissues implanted in severe combined immunodeficient mice respond to gene transfer of a cytokine inhibitor. J Bone Joint Surg Am, 2005-05-01;87(5):1088-97. 2005-05-01 [PMID: 15866974] (IHC, Human)

Bioinformatics

• Gene Symbol: CD68
• Uniprot:
  • AAB25811()