CD55/DAF Antibody (MEM-118)

Images

 
Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) purified, GAM-APC.
Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood leukocytes by mouse monoclonal anti-CD55 antibody MEM-118.
Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) PE-Cy(TM)5.
Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) PE.
Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) Alexa Fluor 488.
Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) biotin / streptavidin-APC.
Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) Alexa Fluor 647.

Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications Flow, Flow-CS, IHC, IHC-P, IP
Clone
MEM-118
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CD55/DAF Antibody (MEM-118) Summary

Immunogen
HPB-ALL human T cell line
Specificity
The antibody MEM-118 recognizes an epitope in SCR4 domain of CD55 (Decay accelerating factor, DAF), a 60-70 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein. CD55 is widely expressed on hematopoietic and on many non-hematopoietic cells; it is weakly present on NK cells. HLDA V; WS Code AS S016
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
CD55
Purity
Size Exclusion Chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:50
  • Flow (Cell Surface)
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CD55/DAF Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS (pH 8.0)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Size Exclusion Chromatography

Alternate Names for CD55/DAF Antibody (MEM-118)

  • CD55 antigen
  • CD55 molecule, decay accelerating factor for complement (Cromer blood group)
  • CD55
  • CR
  • CRdecay accelerating factor for complement (CD55, Cromer blood group system)
  • CROMDAFcomplement decay-accelerating factor
  • DAF
  • decay accelerating factor for complement
  • TC

Background

Avoidance by host tissues of attack by autologous complement proteins is dependent in part on the activities of membrane regulatory factors. One molecule involved in this control is a 70 kD glycoprotein termed decay-accelerating factor (DAF), or CD55. Interruption by CD55 of the complement sequence at an early step in activation effectively halts progression of the cascade and prevents consequent cell injury. In man, CD55 is expressed on the plasma membrane of all cell types that are in intimate contact with plasma complement proteins. This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. CD55 also acts as the receptor for echovirus 7 and related viruses(echoviruses 13, 21, 29 and 33). It is attached to the membrane by a GPI-anchor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD55
Entrez