CD55/DAF Antibody (143-30)

Images

 
Immunocytochemistry/ Immunofluorescence: CD55/DAF Antibody (143-30) [NBP2-44873] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells with CD55/DAF Antibody (143-30) followed by goat anti-Mouse IgG-CF488 ...read more
Flow Cytometry: CD55/DAF Antibody (143-30) [NBP2-44873] - Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells with CD55/DAF Antibody (143-30) followed by goat anti-Mouse IgG-CF488 (Blue) Isotype Control (Red)
Flow (Cell Surface): CD55/DAF Antibody (143-30) [NBP2-44873] - Flow Cytometry: CD55/DAF Antibody (143-30) [Allophycocyanin] [NBP2-47964APC] - A surface stain was performed on HeLa with CD55/DAF Antibody (143-30) ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, Flow-CS, ICC/IF, IHC, IHC-Fr, PAGE
Clone
143-30
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

CD55/DAF Antibody (143-30) Summary

Immunogen
PHA stimulated human PBL
Localization
Cell Surface
Specificity
Recognizes a single chain glycoprotein of 70kDa, identified as CD55 (also known as decay accelerating factor, DAF). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The MAb 143-30 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CD55
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5 - 1.0 ug/ml
  • SDS-Page
  • Flow (Cell Surface)
Application Notes
Hu-chromosome location: 1q32
Immunohistochemistry-Frozen 0.5 - 1 ug/ml for 30 minutes at RT.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for CD55/DAF Antibody (143-30)

  • CD55 antigen
  • CD55 molecule, decay accelerating factor for complement (Cromer blood group)
  • CD55
  • CR
  • CRdecay accelerating factor for complement (CD55, Cromer blood group system)
  • CROMDAFcomplement decay-accelerating factor
  • DAF
  • decay accelerating factor for complement
  • TC

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for CD55/DAF Antibody (NBP2-44873) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47964AF350
Alexa Fluor 405 NBP2-47964AF405
Alexa Fluor 488 NBP2-47964AF488
Alexa Fluor 532 NBP2-47964AF532
Alexa Fluor 594 NBP2-47964AF594
Alexa Fluor 647 NBP2-47964AF647
Alexa Fluor 700 NBP2-47964AF700
Alexa Fluor 750 NBP2-47964AF750
Allophycocyanin NBP2-47964APC
Allophycocyanin/Cy7 NBP2-47964APCCY7
Biotin NBP2-47964B
DyLight 350 NBP2-47964UV
DyLight 405 NBP2-47964V
DyLight 488 NBP2-47964G
DyLight 550 NBP2-47964R
DyLight 594 NBP2-47964DL594
DyLight 650 NBP2-47964C
DyLight 680 NBP2-47964FR
DyLight 755 NBP2-47964IR
FITC NBP2-47964F
HRP NBP2-47964H
Janelia Fluor 549 NBP2-47964JF549
Janelia Fluor 646 NBP2-47964JF646
PE NBP2-47964PE
PE/Atto594 NBP2-47964PEATT594
PE/Cy5.5 NBP2-47964PECY55
PE/Cy7 NBP2-47964PECY7
PerCP NBP2-47964PCP

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