CD55/DAF Antibody (143-30)

Immunocytochemistry/Immunofluorescence: CD55/DAF Antibody (143-30) [NBP2-44873] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells with CD55/DAF Antibody (143-30) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot.

Flow Cytometry: CD55/DAF Antibody (143-30) [NBP2-44873] - Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells with CD55/DAF Antibody (143-30) followed by goat anti-Mouse IgG-CF488 (Blue) Isotype Control (Red)

Flow (Cell Surface): CD55/DAF Antibody (143-30) [NBP2-44873] - Flow Cytometry: CD55/DAF Antibody (143-30) [Allophycocyanin] [NBP2-47964APC] - A surface stain was performed on HeLa with CD55/DAF Antibody (143-30) NBP2-47964APC and a matched isotype control. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin form of this antibody.

• Reactivity: Hu
• Applications: Flow, Flow-CS, ICC/IF, IHC, IHC-Fr
• Clone: 143-30
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CD55/DAF Antibody (143-30) Summary

• Immunogen: PHA stimulated human PBL
• Localization: Cell Surface
• Specificity: Recognizes a single chain glycoprotein of 70kDa, identified as CD55 (also known as decay accelerating factor, DAF). CD55/DAF is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The MAb 143-30 can be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD55
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5 - 1.0 ug/ml
  • Flow (Cell Surface)
• Application Notes: Hu-chromosome location: 1q32
  Immunohistochemistry-Frozen 0.5 - 1 ug/ml for 30 minutes at RT.
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD55/DAF Antibody (143-30)

• CD55 antigen
• CD55 molecule, decay accelerating factor for complement (Cromer blood group)
• CD55
• CR
• CRdecay accelerating factor for complement (CD55, Cromer blood group system)
• CROMDAFcomplement decay-accelerating factor
• DAF
• decay accelerating factor for complement
• TC

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD55
• Uniprot:
  • P08174()
  • P08174()