CD55/DAF Antibody (143-30)

Immunocytochemistry/Immunofluorescence: CD55/DAF Antibody (143-30) [NBP2-44873] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells with CD55/DAF Antibody (143-30) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot.

Flow Cytometry: CD55/DAF Antibody (143-30) [NBP2-44873] - Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells with CD55/DAF Antibody (143-30) followed by goat anti-Mouse IgG-CF488 (Blue) Isotype Control (Red)

• Reactivity: Hu
• Applications: Flow, Flow-CS, ICC/IF
• Clone: 143-30
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CD55/DAF Antibody (143-30) Summary

• Immunogen: PHA stimulated human CD55/DAF (Uniprot: P08174 )
• Localization: Cell Surface
• Specificity: Recognizes a single chain glycoprotein of 70kDa, identified as CD55/DAF. It is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The monoclonal antibody 143-30 may be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD55
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface)
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47964)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for CD55/DAF Antibody (143-30)

• CD55 antigen
• CD55 molecule, decay accelerating factor for complement (Cromer blood group)
• CD55
• CR
• CRdecay accelerating factor for complement (CD55, Cromer blood group system)
• CROMDAFcomplement decay-accelerating factor
• DAF
• decay accelerating factor for complement
• TC

Background

CD55 encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD55
• Uniprot:
  • P08174()
  • P08174()
  • P08174()