CD5 Antibody [Unconjugated]

Images

 
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human CD5 Antigen Affinity-purified ...read more
Simple Western lane view shows lysates of CEM human T-lymphoblastoid cell line, loaded at 0.2 mg/mL. A specific band was detected for CD5 at approximately 93 kDa (as indicated) using 10 µg/mL of Goat Anti-Human ...read more
Human CD5 Antigen Affinity-purified Polyclonal Antibody enhances IL-2 secretion in human T cells in the presence of sub-optimal amounts of Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100) and Human CD28 ...read more
CD5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human CD5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1636) at 5 µg/mL for 3 hours at room ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, Flow, CyTOF-ready, Func, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

CD5 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD5
Arg25-Asn371
Accession # P06127
Specificity
Detects human CD5 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CD5
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Simple Western 10 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Agonist Activity 2-8 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Publications
Read Publication using
AF1636 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD5 Antibody [Unconjugated]

  • CD5 antigen (p56-62)
  • CD5 antigen
  • CD5 molecule
  • CD5
  • LEU1T-cell surface glycoprotein CD5
  • Lymphocyte antigen T1/Leu-1
  • T1

Background

CD5, also known as Leu-1, is a type I transmembrane protein belonging to the scavenger receptor cysteine-rich (SRCR) superfamily. It is expressed on thymocytes, T cells and the B1a subpopulation of B cells. CD5 binds the C-type lectin, CD72, and modulates signaling through the antigen receptors on T and B cells. CD5 has been shown to provide either positive or negative co-stimulatory signals depending on cell type to regulate immune responses.

  1. Ledbetter, J.A. et al. 1985, J. Immunol. 135:2331.
  2. Damle, N.K. et al. 1988, J. Immunol. 140:1753.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD5 Antibody (AF1636)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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