CD5 Antibody (CL12334)

Images

 
Western Blot: CD5 Antibody (CL12334) [NBP3-15133] - Analysis in human cell line MOLT-4.
Immunocytochemistry/ Immunofluorescence: CD5 Antibody (CL12334) [NBP3-15133] - Staining of A-431 cells using the Anti-CD5 monoclonal antibody, showing localization in plasma membrane in green. Microtubule- and nuclear ...read more
Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133] - Analysis in human lymph node and skeletal muscle tissues using NBP3-15133 antibody. Corresponding CD5 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133] - Staining of human chronic lymphocytic leukemia shows strong membranous positivity in tumor cells.
Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133] - Staining of human lymph node shows strong membranous positivity in germinal non-germinal center cells.
Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133] - Staining of human small intestine shows strong membranous positivity in lymphoid cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
CL12334
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CD5 Antibody (CL12334) Summary

Immunogen
This antibody was developed against a synthetic peptide. Actual immunogen sequence is proprietary information.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CD5
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:1000- 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2), 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for CD5 Antibody (CL12334)

  • CD5 antigen (p56-62)
  • CD5 antigen
  • CD5 molecule
  • CD5
  • LEU1T-cell surface glycoprotein CD5
  • Lymphocyte antigen T1/Leu-1
  • T1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CD5