Mouse CD48/SLAMF2 ELISA Kit (Colorimetric) Summary
| Specificity |
Recognizes both recombinant and natural Mouse CD48/SLAMF2. |
| Standard Curve Range |
62.5-4000 pg/ml (example only; lot dependent) |
| Sensitivity |
16.01 pg/ml |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 7.8 % |
| Intra-Assay |
CV% < 3.9 |
| Spike Recovery |
serum (n=3): 81 -87%
supernatant (n=3): 91 -105%
urine (n=3): 81 -87%
|
| Sample Volume |
100 ul |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
CD48 |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage is content dependent. |
Kit Components
|
Components
|
- 1. 96 well microplate coated with Capture Antibody
- 2. Detection Antibody conjugated to HRP
- 3. Standards
- 4. Wash Buffer Concentrate
- 5. Dilution Buffer Concentrate
- 6. Color Reagent A
- 7. Color Reagent B
- 8. Stop Solution
|
Alternate Names for Mouse CD48/SLAMF2 ELISA Kit (Colorimetric)
Background
CD48 is a glycosyl phophatidyl inositol (GPI) anchored cell surface protein also known as Blast1, HuLy m3, BCM1 (in mouse), and OX45 (in rat). CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibrolasts, epithelium and endothelium. CD48 is one of the markers for detecting the defects of GPI anchoring structure on patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2. The CD48 antigen is closely related to the activation antigen, Blast 1.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for CD48/SLAMF2 ELISA Kit (NBP2-80373). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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