CD48/SLAMF2 Antibody (394607) [Phycoerythrin]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD48/SLAMF2 PE-conjugated Monoclonal Antibody (Catalog # FAB3644P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 394607
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

CD48/SLAMF2 Antibody (394607) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD48/SLAMF2
  Gln27-Ser220
  Accession # P09326.2
• Specificity: Detects human CD48/SLAMF2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse CD48 or recombinant human OX40 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD48
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8degrees C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD48/SLAMF2 Antibody (394607) [Phycoerythrin]

• BCM1 surface antigen
• BCM1
• BCM1Leukocyte antigen MEM-102
• BLAST
• BLAST1
• BLAST1TCT.1
• B-lymphocyte activation marker BLAST-1
• CD48 antigen (B-cell membrane protein)
• CD48 antigen
• CD48 molecule
• CD48
• hCD48
• mCD48
• MEM-102
• SLAMF2

Background

CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily molecules (1-3). The human CD48 cDNA encodes a 243 amino acid (aa) precursor that includes a 26 aa signal sequence, a 194 aa mature protein that contains one Ig-like V-type domain and one Ig-like C2-type domain, and a 23 aa C-terminal propeptide (4). A soluble form of CD48 has been detected in the serum of lymphoid leukemia and arthritis patients (5). Human CD48 shares approximately 50% aa sequence identity with mouse and rat CD48. It shares 20%-26% aa sequence identity with comparable regions of human CD2 family members 2B4, CD2, BLAME, CD2F-10, CD84, CD229, CRACC, NTB-A, and SLAM. CD48 is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors (4, 6). Among dendritic cells (DC), CD48 is selectively expressed on circulating myeloid DC and resident bone marrow and thymus DC (7). CD2, 2B4, and heparan sulfate function as CD48 ligands (8-10). CD48 is competent to transduce signals and can also trigger signaling through CD2 or 2B4 (8, 11). CD48-CD2 interactions promote T cell activation and class switching to IgG 2a in B cells (8, 12). High affinity CD48-2B4 interactions can either promote or inhibit NK cell and cytotoxic T cell (CTL) activation (7, 11, 13, 14). In mouse, CD48-2B4 ligation does not directly trigger CTL activity but enhances signaling from the T cell receptor (13). CD48-2B4 mediated inhibition of NK cell activity is distinct from MHC I-restricted mechanisms (15). CD48 expressed on NK cells is coactivating, whereas CD48 expressed on other cell types inhibits NK cell activation (14). Both CD48 expressing and non-expressing cells can be targets of NK cell or CTL-mediated lysis (13, 16).

1. Assarsson, E. et al. (2005) J. Immunol. 175:2045.
2. Bhat, R. et al. (2006) J. Leukocyte Biol. 79:417.
3. Loertscher, R. and P. Lavery (2002) Transpl. Immunol. 9:93.
4. Wong, Y.W. et al. (1990) J. Exp. Med. 171:2115.
5. Smith, G.M. et al. (1997) J. Clin. Immunol. 17:502.
6. Keil, M.J. et al. (2005) Cell 121:1109.
7. Morandi, B. et al. (2005) J. Immunol. 175:3690.
8. Kato, K. et al. (1992) J. Exp. Med. 176:1241.
9. Latchman, Y. et al. (1998) J. Immunol. 161:5809.
10. Ianelli, C.J. et al. (1998) J. Biol. Chem. 273:23367.
11. Messmer, B. et al. (2006) J. Immunol. 176:4646.
12. Gao, N. et al. (2005) J. Immunol. 174:4113.
13. Lee, K-M. et al. (2003) J. Immunol. 170:4881.
14. Lee, K-M. et al. (2006) Blood 107:3181.
15. McNerney, M.E. et al. (2005) Blood 106:1337.
16. Lee, K-M. et al. (2004) J. Exp. Med. 199:1245.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD48/SLAMF2 Antibody (FAB3644P)(1)

Publication using FAB3644P

• Cho D, Shook DR, Shimasaki N Cytotoxicity of activated natural killer cells against pediatric solid tumors. Clin. Cancer Res., 2010-06-11;16(15):3901-9. 2010-06-11 [PMID: 20542985] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: CD48
• Uniprot:
  • P09326.2()