CD47 Antibody (HCD47) [FITC]

Product Discontinued

CD47 Antibody (HCD47) [FITC] Summary

• Immunogen: The immunogen was the full length version of the protein: CD47 (HCD47)
• Specificity: CD47 (HCD47)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD47
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 20 microliter per million cells or 20 microliter per 100 microliter of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2) and 0.2% (w/v) BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD47 Antibody (HCD47) [FITC]

• antigen identified by monoclonal 1D8
• Antigenic surface determinant protein OA3
• CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
• CD47 antigen
• CD47 glycoprotein
• CD47 molecule
• CD47
• IAP
• IAPintegrin associated protein
• Integrin-associated protein
• leukocyte surface antigen CD47
• MER6
• MER6integrin-associated signal transducer
• OA3
• Protein MER6
• Rh-related antigen

Background

CD47 also known as Rh-associated protein, gp42, integrin-associated protein (IAP), and neurophilin, is a 42-52 kD member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. Two splice variants have been described in the cytoplasmic tail, the shorter form is expressed in bone-marrow-derived cells, endothelial cells, and fibroblasts while the longer form is expressed by neural tissues. CD47 expression is widely distributed in hematopoietic cells including thymocytes, T cells, B cells, monocytes, platelets, and erythrocytes as well as epithelial cells, endothelial cells, fibroblasts, and neural tissues. CD47 functions as an adhesion molecule and thrombospondin receptor and is non-covalently associated with beta3 integrins CD51/CD61, CD41/CD61. Thrombospondin is a ligand for CD47; in the absence of CD47 mice show defects in host defense and beta3 integrin-dependent ligand binding, migration, and cellular activation. CD47 is also part of the Rh complex on erythrocytes. The HCD47 antibody recognizes human CD47 and has been shown to be useful for flow cytometry.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD47