CD47 Antibody (472603) [Unconjugated]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD47 Monoclonal Antibody (Catalog # MAB4670, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

In afunctional flowcytometry test,biotinylated recombinant Human SIRP alpha /CD172a (100ng/mL, Catalog #4546-SA) binds to Human CD47-transfected HEK293 human embryonic kidney cell line (black dotted line). Binding is completely blocked (orange histogram) by 2.5 μg/mL of Mouse Anti-Human CD47 MonoclonalAntibody (Catalog # MAB4670). Mouse IgG1 isotype (Catalog #MAB002) at 2.5 μg/mL was used as a control (blue line).Cells were stained with Streptavidin-APC (Catalog # F0050).

• Reactivity: Hu
• Applications: Flow, B/N, CyTOF-ready
• Clone: 472603
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CD47 Antibody (472603) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human CD47
  Gln19-Pro139
  Accession # Q08722
• Specificity: Detects human CD47. Stains human CD47 transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD47
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Blockade of Receptor-ligand Interaction
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD47 Antibody (472603) [Unconjugated]

• antigen identified by monoclonal 1D8
• Antigenic surface determinant protein OA3
• CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
• CD47 antigen
• CD47 glycoprotein
• CD47 molecule
• CD47
• IAP
• IAPintegrin associated protein
• Integrin-associated protein
• leukocyte surface antigen CD47
• MER6
• MER6integrin-associated signal transducer
• OA3
• Protein MER6
• Rh-related antigen

Background

CD47, also known as integrin-associated protein, is a ubiquitous 50 kDa multipass transmembrane protein with a single IgV-like domain at its N-terminus. CD47 binding to SIRP alpha prevents the phagocytic engulfment of viable cells. Thrombospondin interaction with CD47 on T cells reduces T cell proliferation and inflammatory reactions. Alternate splicing generates isoforms with truncated cytoplasmic domains. Within the N-terminal ECD, human CD47 shares 63% aa sequence identity with mouse and rat CD47.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD47 Antibody (MAB4670)(2)

Publications using MAB4670

• Morse, M;Rodriguez, X;DeLaRosa, E;Rodriguez, S;Shanil, J;Sinha, S; SIRP? modulates effector differentiation of human CD8 T Cells under suboptimal TCR stimulation: implications for immune homeostasis and autoimmunity bioRxiv : the preprint server for biology 2025-07-14 [PMID: 40791325] (Neutralization, Human)
• X Ye, X Wang, R Lu, J Zhang, X Chen, G Zhou CD47 as a potential prognostic marker for oral leukoplakia and oral squamous cell carcinoma Oncol Lett, 2018-04-17;15(6):9075-9080. 2018-04-17 [PMID: 29805639] (IHC, Inhibition, Human)

Bioinformatics

• Gene Symbol: CD47
• Uniprot:
  • Q08722()