CD46 Antibody (122.2)

Flow Cytometry: CD46 Antibody (122.2) [NBP2-25222] - FCM staining of human PBMCs using CD46 Antibody (122.2).

• Reactivity: Hu
• Applications: Flow, ICC/IF, ICC/IF
• Clone: 122.2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CD46 Antibody (122.2) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-33159)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Additional Information: Clone 122.2 was used by HLDA to establish CD designation.
• Immunogen: Recombinant human CD46 protein (Uniprot: P15529)
• Localization: Cell surface
• Marker: T-Cell Marker
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD46
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5-1ug/ml
• Application Notes: Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD46 Antibody (122.2)

• AHUS2
• CD46 antigen
• CD46 molecule, complement regulatory protein
• CD46
• MCP
• membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen)
• MIC10
• TLXcomplement regulatory protein
• trophoblast leucocyte common antigen
• Trophoblast leukocyte common antigen
• trophoblast-lymphocyte cross-reactive antigen

Background

CD46 acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. CD46 acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD46
• Uniprot:
  • NP_002380()
  • P15529(Human)