CD45RA Antibody (OX-33) [FITC] - (B Cells only)

Flow Cytometry: CD45RA Antibody (OX-33) [FITC] - - (B Cells only) [NB100-63846] - Figure A. A647 conjugated mouse anti rat CD45 and FITC conjugated mouse IgG1 isotype control. Figure B. A647 conjugated mouse anti rat CD45 and FITC conjugated mouse anti rat CD45RA. All experiments performed on red cell lysed rat blood gated on mononuclear cells.

Product Discontinued

CD45RA Antibody (OX-33) [FITC] - (B Cells only) Summary

• Immunogen: Purified Rat spleen L-CA
• Localization: Membrane; single pass type I membrane protein.
• Specificity: NB100-65000 is directed against a high molecular weight band of the leucocyte common antigen (LCA). The MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labelling in bone marrow and none on thymocytes (2).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10
• Application Notes: Membrane permeabilisation is required for Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A purified

Alternate Names for CD45RA Antibody (OX-33) [FITC] - (B Cells only)

• B220
• CD45 antigen
• CD45R
• CD45RA
• EC 3.1.3.48
• L-CA
• LY5
• protein tyrosine phosphatase, receptor type, C
• receptor-type tyrosine-protein phosphatase C
• T200 glycoprotein
• T200 leukocyte common antigen
• T200receptor type, c polypeptide

Background

CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRC
• Uniprot:
  • P04157(Rat)